We narrowed to 5,097 results for: puc

Showing: 2921 - 2960 of 5097 results

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pU6-Cas13bt1-non-targeting (pJK2)

Plasmid

#229054

Purpose

Cloning backbone for Cas13bt1 crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-Cas13bt1-non-targeting (pEZ25)

Plasmid

#229055

Purpose

Cloning backbone for Cas13bt1 crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-RanCas13b-non-targeting (pJK6)

Plasmid

#229056

Purpose

Cloning backbone for RanCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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phU1-RanCas13b-non-targeting (pEZ45)

Plasmid

#229057

Purpose

Cloning backbone for RanCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW00015

Plasmid

#222446

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

VP64-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00014

Plasmid

#222445

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

VP16-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW110034

Plasmid

#222443

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

LoxP-mCherry

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pNHA10

Plasmid

#223554

Purpose

TurboID expression

Depositor

François-Xavier Campbell-Valois

Article

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

TurboID

Tags

Myc

Expression

Bacterial

Promoter

rpsM

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAN102

Plasmid

#220045

Purpose

Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

crRNA; Cas6

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT346

Plasmid

#204505

Purpose

5S rDNA used as a probe for chromosome FISH in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

5S rDNA (C. elegans)

Use

Other

Promoter

T7

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1037

Plasmid

#169616

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1108_Tier3(Lenti_inverse)

Plasmid

#169663

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS1035

Plasmid

#169614

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PhCMV

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Expression

Mammalian

Promoter

PPGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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FBXO7_Halo_C_allele

Plasmid

#178158

Purpose

Donor vector for endogenous tagging of human FBXO7 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FBXO7 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SETX_Halo_C_allele

Plasmid

#178169

Purpose

Donor vector for endogenous tagging of human SETX at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (SETX Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ITM2B_Halo_C_allele

Plasmid

#178160

Purpose

Donor vector for endogenous tagging of human ITM2B at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ITM2B Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GCH1_Halo_N_allele

Plasmid

#178159

Purpose

Donor vector for endogenous tagging of human GCH1 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (GCH1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBL-2

Plasmid

#162769

Purpose

Minimal backbone with AmpR

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Synthetic Biology; Molecular cloning

Promoter

no

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TC811

Plasmid

#164893

Purpose

TRE::YFP, inducible YFP expression when co-express with rtTA

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

YFP

Expression

Worm

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pISKP1-TIR1

Plasmid

#165061

Purpose

Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-313 (attR4-attR3)

Plasmid

#161898

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP105

Plasmid

#165060

Purpose

Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID9L104

Plasmid

#165054

Purpose

Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC_cherry donor_Rosa target (pE002)

Plasmid

#149348

Purpose

Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC_cherry donor_Polb target (pE038)

Plasmid

#149349

Purpose

Replace donor vector for cherry insertion using the human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQDKN0332

Plasmid

#162314

Purpose

Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

SP6prom-EO1-sfGFP-HiBiT

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQDKN0329

Plasmid

#162315

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease-HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0283

Plasmid

#162288

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0282

Plasmid

#162287

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0244

Plasmid

#162282

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPQD0KN0024

Plasmid

#162281

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV23

Plasmid

#128887

Purpose

Position 4 module- STOP (stop codon)

Depositor

Gilles Charvin

Article

Goulev et al PLoS One. 2019 Aug 28;14(8):e0220694. do

Insert

STOP

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL7006

Plasmid

#136945

Purpose

Malachite green aptamer, bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Promoter

J23119

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK010

Plasmid

#128227

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAK011

Plasmid

#128228

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 5

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 5

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 5,097 results for: puc