Addgene

We narrowed to 12,997 results for: SHI

Showing: 3651 - 3700 of 12997 results

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3243 pSHLox Hox 11 L2

Plasmid

#8762

Depositor

Stanley Korsmeyer

Article

Shirasawa et al Nat Med 1997 Jun;3(6):646-50.

Insert

Hox11 L2 (Tlx3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pProm1_BCD20-GFP

Plasmid

#80651

Purpose

Promoter1 with BCD20-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm1_BCD21-GFP

Plasmid

#80652

Purpose

Promoter1 with BCD21-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P279L

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRET.IIS.IRES-EGFP

Plasmid

#1838

Depositor

Philip Leder

Article

Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
MKT074

Plasmid

#231997

Purpose

MicF RNA toehold switch with NanoLuc complementation peptide reporter

Depositor

Melissa Takahashi

Article

Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

Insert

MicF RNA toehold switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDC.100

Plasmid

#222224

Purpose

Acceptor plasmid for yeast retron-Eco1 ncRNA variant libraries.

Depositor

Seth Shipman

Article

Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMK805

Plasmid

#207139

Purpose

Assembled Tn10 with ptac promoter driving gfp

Depositor

Nicholas Shikuma

Article

Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

Insert

Tn10-Ptac-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB303-MpIGPDm

Plasmid

#188555

Purpose

Binary vector for MpIGPDm and mALS as selective markers in Marchantia polymorpha (igpd mutants)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

MpIGPDm and mALS

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-YP_001498439

Plasmid

#198370

Purpose

Express YP_001498439 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001498439

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mTurquoise2-Scarlet-I

Plasmid

#193558

Purpose

Expresses mTurquoise2-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-mScarlet-I

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C-SWAT SMASh

Plasmid

#182523

Purpose

C-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK29-wzzpHS2

Plasmid

#172971

Purpose

pWSK29 with S. flexneri wzzpHS2 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzPHS2 gene from Shigella flexneri

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK29-wzzBSF

Plasmid

#172967

Purpose

pWSK29 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Shigella flexneri

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CMYA5tnC/pDEST26-N-FLAG

Plasmid

#98130

Purpose

Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector

Depositor

Rita Shiang

Insert

CMYA5 (CMYA5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TTN/pAS2-1

Plasmid

#98127

Purpose

titin in DNA-binding domain (BD) vector, used for yeast-two hybrid

Depositor

Rita Shiang

Insert

TTN (TTN Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSKB3.EG10680

Plasmid

#11436

Depositor

Sung-Hou Kim

Article

Shin et al Acta Crystallogr D Biol Crystallogr. 200

Insert

OsmC (osmC E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
p070 pCS xTRPS1 delN delC320

Plasmid

#11041

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Tags

Expression

Mutation

aa806-952 of xTRPS1

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
p062 pCS xTRPS1 deltaN

Plasmid

#11034

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 deltaN (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Tags

Expression

Mutation

aa806-1272 of xTRPS1

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V134L

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 V33A Cas2

Plasmid

#80093

Purpose

Encodes Cas1 V33A and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V33A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 V190I Cas2

Plasmid

#80094

Purpose

Encodes Cas1 V190I and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V190I

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 P202Q Cas2

Plasmid

#80096

Purpose

Encodes Cas1 P202Q and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P202Q

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 P212L Cas2

Plasmid

#80097

Purpose

Encodes Cas1 P212L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P212L

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 P282L Cas2

Plasmid

#80098

Purpose

Encodes Cas1 P282L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P282L

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 P294H Cas2

Plasmid

#80099

Purpose

Encodes Cas1 P294H and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P294H

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 Cas2 T72I

Plasmid

#80100

Purpose

Encodes Cas1 and Cas2 T72I

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

T72I

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 Cas2 G74A

Plasmid

#80101

Purpose

Encodes Cas1 and Cas2 G74A

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

G74A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG mito-RCaMP1h

Plasmid

#105013

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

2xCLEAR

Available since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG G-CEPIA1er

Plasmid

#105012

Purpose

ER calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

G-CEPIA1er

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IB-pSu9-HaloTag7-mGFP

Plasmid

#184905

Purpose

Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

Presequence of Fo-ATPase subunit 9

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Smad7-HA

Plasmid

#11733

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV500 empty vector

Plasmid

#33348

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mTurquoise2-ShadowY

Plasmid

#193557

Purpose

Expresses mTurquoise2-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-ShadowY

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-M.CviQIX (NDel15)

Plasmid

#198357

Purpose

Express M.CviQIX (NDel15) in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviQIX

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-hG9a-ΔSET

Plasmid

#33026

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the SET domain; contains aa 1-758

Promoter

CMV

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-Sec31a #891#342

Plasmid

#42110

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
CMV566 empty vector

Plasmid

#33349

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only
PB-NLS-ZapCV2

Plasmid

#203664

Purpose

Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2 Zinc sensor

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
poRFP-oGFP

Plasmid

#154130

Purpose

Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

orthogonal gfp
orthogonal rfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UnaG

Plasmid

#203485

Purpose

For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.436

Plasmid

#184953

Purpose

Express -Eco1 RT and ncRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: ncRNA(wt) and RT

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-OT

Plasmid

#184596

Purpose

For expression of a fluorescent sensor for oxytocin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-M.CviPII (NDel15)

Plasmid

#198356

Purpose

Express M.CviPII (NDel15) in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

M.CviPII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Sec31a #861#640

Plasmid

#42123

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Showing: 3651 - 3700 of 12997 results

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