We narrowed to 44,084 results for: cha

1. pCR8/GW/TOPO-ETV1

   Plasmid

   #98623

   Purpose

   Gateway entry TOPO TA cloning vector for ETV1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ETV1 (Etv1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCR8/GW/TOPO-ETV6

   Plasmid

   #98625

   Purpose

   Gateway entry TOPO TA cloning vector for ETV6

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ETV6 (Etv6 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR8/GW/TOPO-ELK3

   Plasmid

   #98622

   Purpose

   Gateway entry TOPO TA cloning vector for ELK3

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK3 (Elk3 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   E167K

   Promoter

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCR8/GW/TOPO-ELF1

   Plasmid

   #98627

   Purpose

   Gateway entry TOPO TA cloning vector ELF1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELF1 (Elf1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMiR-CIP2A(partial)

   Plasmid

   #53705

   Purpose

   Luciferase reporter assay for microRNA binding on CIP2A coding sequence

   Depositor

   Edward Chan

   Article

   Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAH33

   Plasmid

   #226850

   Purpose

   To constitutively overexpress Opa1 from the safe harbor locus Rosa26 in MEF

   Depositor

   Luke Chao

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   Opa1 (Opa1 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET303C-hSOD1 A4V/C6S

   Plasmid

   #139667

   Purpose

   Expression plasmids containing human A4V/C6S SOD1

   Depositor

   Svetlana Antonyuk

   Article

   Chantadul et al Commun Biol. 2020 Mar 5;3(1):97. doi: 10

   Insert

   Superoxide dismutase-1 (SOD1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   change alanine 4 to valine, change cysteine 6 to …

   Promoter

   T7

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLgw NKX2-5-V5-EcoDam

   Plasmid

   #98597

   Purpose

   Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR8/GW/TOPO-ELK1Y158A

   Plasmid

   #98628

   Purpose

   Gateway entry TOPO TA cloning vector ELK1 Y158A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 Y158A (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Y158A

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR8/GW/TOPO-ETS1

    Plasmid

    #98621

    Purpose

    Gateway entry TOPO TA cloning vector for ETS1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ETS1 (Ets1 Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-ASAP3Kv-WPRE

    Plasmid

    #132332

    Purpose

    expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3Kv
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-EF1a-DIO-ASAP3-WPRE

    Plasmid

    #132318

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MCUabaa-Flag-PLYS1

    Plasmid

    #236786

    Purpose

    MCU-MCUB fusion in abaa pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MCUabab-Flag-pLYS1

    Plasmid

    #236788

    Purpose

    MCU-MCUB fusion in abab pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLgw EcoDam-V5-ELK1

    Plasmid

    #98604

    Purpose

    Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP Cav2.2 HA D122A pcDNA3

    Plasmid

    #206120

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cav2.2 HA E1658A (EIVA) pcDNA3

    Plasmid

    #206114

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MCUabbb-Flag-PLYS1

    Plasmid

    #236787

    Purpose

    MCU-MCUB fusion in abbb pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDual

    Plasmid

    #207883

    Purpose

    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

    Depositor

    David Edgell

    Article

    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

    Inserts

    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Leucine 212 changed to proline, leucine 434 in th…

    Promoter

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid

    #206082

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid

    #206066

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid

    #206096

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV/B-actin

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEP4 TAPBPR-TM-TN6

    Plasmid

    #178650

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    E205K, R207E, Q209S, Q272S, switched transmembran…

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

    Plasmid

    #105681

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

    Plasmid

    #105680

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid

    #98598

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid

    #98599

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMiR-CIP2A-stop-mut

    Plasmid

    #53713

    Purpose

    Luciferase reporter assay for CIP2A with mutated firefly stop codon

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on the stop codon of firefly luciferase …

    Promoter

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pAAV-EF1a-DIO-ASAP3Kv-WPRE

    Plasmid

    #132330

    Purpose

    expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3KV
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Lenti-mCherry-G12V-KRAS-IRES-Blast

    Plasmid

    #153336

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    mCherry-G12V-KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Lenti-mCherry-WT-KRAS-IRES-Blast

    Plasmid

    #153335

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    mCherry-WT-KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLgw ELK4-V5-EcoDam

    Plasmid

    #98607

    Purpose

    Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSITEP-puro-shWRN1

    Plasmid

    #125783

    Purpose

    DOX-inducible expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLKO.1-puro-shRFP

    Plasmid

    #125778

    Purpose

    constitutive expression of a short-hairpin RNA targeting RFP

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shRFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSITEP-puro-shWRN1-C911

    Plasmid

    #125784

    Purpose

    a seed-matched control for pRSITEP-puro-shWRN1

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1-C911 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRSITEP-puro-shWRN2

    Plasmid

    #125785

    Purpose

    DOX-inducible expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN2 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRSITEP-puro-shWRN2-C911

    Plasmid

    #125786

    Purpose

    a seed-matched control for pRSITEP-puro-shWRN2

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN2 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLKO.1-puro-shWRN1

    Plasmid

    #125781

    Purpose

    constitutive expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid

    #206121

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLKO.1-puro-shWRN2

    Plasmid

    #125782

    Purpose

    constitutive expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN2 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pXPR_BRD003-sgWRN1

    Plasmid

    #125771

    Purpose

    constitutive expression of a guide RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgWRN1 (WRN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLKO.1-puro-shPSMD2

    Plasmid

    #125779

    Purpose

    constitutive expression of a short-hairpin RNA targeting human PSMD2 (RNAi positive control)

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shPSMD2 (PSMD2 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pXPR_BRD003-sgWRN2

    Plasmid

    #125772

    Purpose

    constitutive expression of a guide RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgWRN2 (WRN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pXPR_BRD051-sgCh2-2

    Plasmid

    #125775

    Purpose

    constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgCh2-2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pXPR_BRD003-sgWRN3

    Plasmid

    #125773

    Purpose

    constitutive expression of a guide RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgWRN3 (WRN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMiR-CIP2A-miR-375-mutA-E

    Plasmid

    #53754

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site A-E (refer to ci…

    Promoter

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only