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We narrowed to 5,960 results for: plasmid dna

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Showing: 1461 - 1480 of 5960 results

  1. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid
    #101183
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    M694A/Q695A/H698A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101183-plasmid-map-sequence-id-194032

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  2. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid
    #101180
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/Q926A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101180-plasmid-map-sequence-id-194005

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  3. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  4. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid
    #101231
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101231-plasmid-map-sequence-id-197986

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  5. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid
    #101229
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    101229-plasmid-map-sequence-id-197216

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  6. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid
    #101208
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A and Q695A
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101208-plasmid-map-sequence-id-198110

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  15. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid
    #169733
    Purpose
    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169733-plasmid-map-sequence-id-334140

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  16. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid
    #169734
    Purpose
    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169734-plasmid-map-sequence-id-334141

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  17. pPSU2

    Plasmid
    #89566
    Purpose
    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    Expression
    Bacterial
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89566-plasmid-map-sequence-id-177206

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  18. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid
    #169735
    Purpose
    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169735-plasmid-map-sequence-id-334142

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  19. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169743
    Purpose
    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    mScarlet-I
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169743-plasmid-map-sequence-id-334355

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  20. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169737
    Purpose
    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169737-plasmid-map-sequence-id-334143

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Showing: 1461 - 1480 of 5960 results