We narrowed to 17,387 results for: puro

1. Ki67∆FHA-mNeonGreen-IRESpuro2

   Plasmid

   #183739

   Purpose

   Expresses Ki67∆FHA-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67_lacking FHA (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking FHA)

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV puro let-7 sponge

   Plasmid

   #29766

   Depositor

   Phil Sharp

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

   Insert

   let-7 sponge
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2011

   Availability

   Academic Institutions and Nonprofits only

3. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

   Plasmid

   #183741

   Purpose

   Expresses Ki67 (8 repeats+LR)-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67 LR domain + 8 Ki-67 repeats

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-DCX-TurboID-V5-puro

   Plasmid

   #190742

   Purpose

   A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet-pLKO-puro shKRAS

   Plasmid

   #116871

   Purpose

   Tet-inducible suppression of KRAS

   Depositor

   William Hahn

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   shKRAS (KRAS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-puro-Flag-mMcl-1

   Plasmid

   #32982

   Depositor

   Joseph Opferman

   Article

   Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

   Insert

   Mcl-1 (Mcl1 Mouse)
   

   Use

   Retroviral

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pWPI-IRES-Puro-Ak-ACE2

   Plasmid

   #154985

   Purpose

   Lentiviral vector expressing Human ACE2

   Depositor

   Sonja Best

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

   Plasmid

   #211969

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

   Plasmid

   #211968

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBpuro-4493NES(Booster-PKA)

    Plasmid

    #184709

    Purpose

    PKA biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti X2 Puro DEST (w16-1)

    Plasmid

    #17296

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-mNeon-Puro-TOMM20

    Plasmid

    #207790

    Purpose

    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-mStayGold-Puro-CLTC

    Plasmid

    #227314

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO‐puro‐2A‐mdx4cv‐GFP

    Plasmid

    #187053

    Purpose

    fluorescent reporter for adenine base editing of mdx4cv point mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti SpBsmBI sgRNA Puro

    Plasmid

    #62207

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES LifeAct-GFP puro3

    Plasmid

    #64357

    Purpose

    Bicistronic expression of LifeAct-GFP fusion and puro3 resistance gene

    Depositor

    Matthieu Piel

    Insert

    LifeAct-GFP
    

    Use

    Tags

    IRES-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pUC118-FLIP-FlpE-Puro

    Plasmid

    #84539

    Purpose

    a mock vector for generating FLIP-FlpE targeting vectors

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    FLIP-FlpE - Puro
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-puro-EGFP-FAK

    Plasmid

    #38194

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    PTK2 protein tyrosine kinase 2 (Ptk2 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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