We narrowed to 17,203 results for: puro

1. V51 pIRESpuro-GLUE (pGLUE)

   Plasmid

   #15100

   Purpose

   tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Type

   Empty backbone

   Use

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro_CMV_NES-Caprola_on-mEGFP

   Plasmid

   #194694

   Purpose

   CMV driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

   Plasmid

   #194695

   Purpose

   CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLenti-CMV-puro-2A-TEVp

   Plasmid

   #99610

   Purpose

   Lentivirus expressing puromycin resistance gene and TEV protease

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   Puromycin resistance (PAC gene)
   TEV protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-Cdt1ΔCy-mCherry-Puro

   Plasmid

   #193764

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   Cdt1ΔCy-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-ND-Cdt1-mCherry-Puro

   Plasmid

   #193766

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-puro/Flag PD-L1 N200Q

   Plasmid

   #121451

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N200Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPOTv6-puro-3Ty:tagRFPt:3Ty

   Plasmid

   #201089

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   tagRFPt
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMMP-MCS-IRES-Puro

    Plasmid

    #36952

    Depositor

    Bill Sugden

    Article

    Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLIX403-iRFP-ccdB-Puro

    Plasmid

    #158566

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWPI-IRES-Puro-Ak-TMPRSS2

    Plasmid

    #154986

    Purpose

    Lentiviral vector expressing Human TMPRSS2

    Depositor

    Sonja Best

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid

    #102909

    Purpose

    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe puro IkB alpha M

    Plasmid

    #12332

    Depositor

    Inder Verma

    Insert

    IkB alpha M (Nfkbia Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant-negative mutant. Many signal transductio…

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLD-puro-Cc-TARDBP-A315T_VA

    Plasmid

    #141329

    Purpose

    Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-A315T in mammalian cells

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-A315T (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

    Expression

    Mammalian

    Mutation

    A315T

    Promoter

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP

    Plasmid

    #102797

    Purpose

    Retrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroR

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Inserts

    sgRNA
    EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H231L

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. aMHC-puro rex-neo

    Plasmid

    #21230

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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