Addgene

We narrowed to 4,587 results for: LUM

Showing: 1481 - 1500 of 4587 results

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pDisplay sHRPa-KDEL

Plasmid

#73149

Purpose

Large fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-KDEL

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1-FKBP

Plasmid

#108129

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Use

Tags

Lamp1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA Hygro

Plasmid

#167907

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

NLS (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Use

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pSB700 lenti gRNA iRFP713_zhang2.0

Plasmid

#167916

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-EXOB-11

Plasmid

#197688

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EXOB enhancer-blocking insulator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Ufm1 WT

Plasmid

#133865

Purpose

Expression of human wild type UFM1

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 (UFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-FRISZ

Plasmid

#176887

Purpose

Far-red fluorescent zinc indicator FRISZ in pDisplay

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Use

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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phage UbiC SNAP-KDEL

Plasmid

#104982

Purpose

Lentiviral vector expressing SNAP-KDEL for ER labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

SNAP-KDEL

Use

Lentiviral

Tags

Secretion signal

Expression

Bacterial and Mammalian

Mutation

Promoter

UbiC

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-p27Luc

Plasmid

#23047

Depositor

William Kaelin

Article

Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

Insert

p27 (CDKN1B Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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hPDI1-18-508_C400S_C56S-pFLAG-CMV1

Plasmid

#31383

Depositor

David Ron

Article

Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

Insert

PDI1A (P4HB Human)

Use

Tags

FLAG M2

Expression

Mammalian

Mutation

C-terminal cysteines of both active sites mutated…

Promoter

Available since

May 1, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S

Plasmid

#108128

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb Wnt3 promoter-EGFP

Plasmid

#203792

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

EGFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

4kb Wnt3 promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Hygro

Plasmid

#167859

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_mcherry

Plasmid

#167862

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47732:PtFCP:ShBle

Plasmid

#104893

Purpose

Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

ShBle

Use

Phaeodactylum; golden gate assembly

Tags

PtFCP promoter and terminator

Expression

Mutation

Promoter

Available since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Puro

Plasmid

#167871

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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