We narrowed to 4,616 results for: pcr

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBP-T7RNAP

Plasmid

#74096

Purpose

T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-3HaloTag

Plasmid

#179828

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-mCherry-kanMX6

Plasmid

#89068

Purpose

genomic GBP-mCherry epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP-mCherry

Expression

Yeast

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Myc:mNG:3Myc

Plasmid

#179803

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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human PNP non-tagged

Plasmid

#64199

Purpose

Expression of native human PNP (non-tagged)

Depositor

Vern Schramm

Article

Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3Ty:mScarletI:3Ty

Plasmid

#201090

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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punc-119c

Plasmid

#21595

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119 (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pPL5618_pUG_MtxR

Plasmid

#60929

Purpose

PCR template vector for generating methotrexate resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Dihydrofolate reductase (DHFR Human)

Use

Loxp flanked deletion cassette template

Mutation

L22F F31S

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSN007

Plasmid

#102440

Purpose

For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

Depositor

Alejandro Chavez, George Church

Article

Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

Insert

7SK paired gRNA insert

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1354

Plasmid

#69490

Purpose

Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

Depositor

Jordan Ward

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope

Use

CRISPR; Template to generate cassettes for -media…

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCCM935

Plasmid

#58202

Purpose

Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

unc-22 sgRNA

Expression

Worm

Promoter

U6

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pPOTv6-puro-3Myc:mScarletI:3Myc

Plasmid

#179804

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Fret-Stop-Fret TOPO

Plasmid

#22774

Depositor

Tyler Jacks

Article

Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

Insert

fret-STOP-fret

Expression

Mammalian

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG2(1-10)

Plasmid

#190209

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen2(1-10)

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template1-3XMyc-Spacer

Plasmid

#176208

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template1-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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punc-119cR

Plasmid

#21596

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119:mCherry (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Tags

mCherry

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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