We narrowed to 21,668 results for: Tes

1. LTR5HS S. pyogenes scaffold CARGO array

   Plasmid

   #191316

   Purpose

   Contains an array of guide RNAs targeting LTR5Hs elements

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCiVSP(C363S)-SD64TF

   Plasmid

   #80334

   Purpose

   Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

   Depositor

   Yasushi Okamura

   Article

   Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

   Insert

   Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   Changed Cysteine 363 to Serine

   Promoter

   SP6

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-RanWT-mRFP1-polyA

   Plasmid

   #59750

   Purpose

   Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran-mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5M-PR-A

   Plasmid

   #89124

   Purpose

   mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-A (PGR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSV Sport SREBP-1c dom neg

   Plasmid

   #8885

   Depositor

   Bruce Spiegelman

   Article

   Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

   Insert

   SREBP-1c (Srebf1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y305A amino acid substitution in the active versi…

   Promoter

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-LBR-EGFP-polyA

   Plasmid

   #59748

   Purpose

   Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-EGFP-ELYS-polyA

   Plasmid

   #59746

   Purpose

   Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   EGFP-ELYS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-LBR-mRFP1-polyA

    Plasmid

    #59749

    Purpose

    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-mRFP1
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    aa 1-249 of LBR

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSGKP_AcsgRNA

    Plasmid

    #203807

    Purpose

    pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1- Ncad-GFP

    Plasmid

    #35676

    Depositor

    James Jontes

    Article

    Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

    Insert

    Ncad (cdh2 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MBP-TEVpCS-ADGRE3 CTFdeltaTA-FLAG

    Plasmid

    #217724

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE3 (ADGRE3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-344

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

    Plasmid

    #217691

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE3 (ADGRE3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-338

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

    Plasmid

    #217690

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE2 (ADGRE2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-517

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG

    Plasmid

    #217723

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE2 (ADGRE2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-523

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJG80

    Plasmid

    #89282

    Purpose

    Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

    Depositor

    Daniel Voytas

    Article

    Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS NL4-3 envFS eGFP Δnef/U3

    Plasmid

    #101346

    Purpose

    Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta nef/LTR

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTrcHisA-rat-PTP1B

    Plasmid

    #35987

    Depositor

    Anna Huttenlocher

    Article

    Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

    Insert

    protein tyrosine phosphatase 1B (Ptpn1 Rat)
    

    Use

    Tags

    His and Xpress Epitope Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

    Plasmid

    #217689

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE1 (EMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-584

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCB6HindIII-rat FcRn

    Plasmid

    #121671

    Purpose

    Expresses rat neonatal Fc receptor in mammalian cells

    Depositor

    Pamela Bjorkman

    Article

    Tesar et al Traffic. 2006 Sep;7(9):1127-42.

    Insert

    rat neonatal Fc receptor (Fcgrt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid

    #74368

    Purpose

    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    yeGFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGRR-r11-r10

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV 4xSTAR-mTFP-NLS-blast

    Plasmid

    #136257

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hME-pOX

    Plasmid

    #178181

    Purpose

    Produce cRNA that expresses a human MCU-EMRE fusion protein in Xenopus oocytes

    Depositor

    Ming-Feng Tsai

    Article

    Rodriguez et al STAR Protoc. 2021 Nov 23;2(4):100979. do

    Insert

    human MCU-EMRE fusion protein
    

    Use

    Tags

    1D4

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHD-mW-attPCC-FRT (pTL717)

    Plasmid

    #115158

    Purpose

    Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    mW attP-CC FRT
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTrc99-Rat FABP2

    Plasmid

    #13578

    Depositor

    Martin Scanlon

    Article

    Velkov et al J Biol Chem. 2005 May 6. 280(18):17769-7

    Insert

    intestinal fatty acid binding protein (Fabp2 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pSCALPS_miR-146aSponge_mCherry_GFP

    Plasmid

    #149718

    Purpose

    Lentiviral vector expressing a miR-146a decoy sponge

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    14X miR-146a binding sites
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G to T mutation in position 75 of the insert

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge