We narrowed to 13,732 results for: TIM

1. pG0A0-1-1-PBAD-dCas9

   Plasmid

   #208815

   Purpose

   Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

   Depositor

   Marc Erhardt

   Article

   Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

   Insert

   Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinsoe-inducible promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-His-E1GFP

   Plasmid

   #233885

   Purpose

   Bacterial Expression of recombinant His-tagged E1GFP

   Depositor

   Francesco Cardarelli

   Article

   De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

   Insert

   E1GFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   T65S and T203Y compared to EGFP

   Promoter

   T7 promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS413-TEF1pr-CFAPorig

   Plasmid

   #221092

   Purpose

   FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS413-TEF1pr-NFAPorig

   Plasmid

   #221091

   Purpose

   FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS415-TEF1pr-NFAPorig

   Plasmid

   #221086

   Purpose

   FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS415-TEF1pr-CFAPorig

   Plasmid

   #221087

   Purpose

   FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDIV302

   Plasmid

   #204973

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   nat1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PTet-F30-Pepper aptamer-UTR1-mTurq2

   Plasmid

   #227645

   Purpose

   pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

   Depositor

   Richard Murray

   Article

   Kapasiawala et al bioRxiv 2023.03.22.533877

   Insert

   F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUB_SM-RLuc0---MS2

   Plasmid

   #210572

   Purpose

   Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Spaghetti monster (FLAG)-nonoptimal Renilla luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Spaghetti monster (FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   UBC human

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUB_SM-RLuc100---MS2

    Plasmid

    #210573

    Purpose

    Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal Renilla luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1_SM-FLuc0-malat1

    Plasmid

    #210580

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1_SM-FLuc100-malat1

    Plasmid

    #210581

    Purpose

    Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUCmini-UGCG-EGFP

    Plasmid

    #225878

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSETA-qmTQ2-glucose

    Plasmid

    #225252

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-glucose
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSETA-qmTQ2-cAMP

    Plasmid

    #225249

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. A99-ELP

    Plasmid

    #170760

    Purpose

    A99 laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    A99-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AG73-ELP

    Plasmid

    #170762

    Purpose

    AG73 laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    AG73-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSpyA-GFP

    Plasmid

    #220189

    Purpose

    sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pEM7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpyD-GFP

    Plasmid

    #220192

    Purpose

    sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG28

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMRS-GFP

    Plasmid

    #220197

    Purpose

    mini-Tn7 GFP expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    promoterless

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMJ074

    Plasmid

    #215654

    Purpose

    pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143

    Depositor

    Brian Pfleger

    Article

    Jones et al Appl Microbiol Biotechnol. 2021 Feb;105(

    Insert

    Yellow fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    10x histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mCherry-C1-ORP5deltaTMD-FKBP

    Plasmid

    #220073

    Purpose

    expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL5deltaTMD (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deltaTMD

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-C2-DR

    Plasmid

    #216266

    Purpose

    Dox-responsive module fused with EGFP

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    Codon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJH4506

    Plasmid

    #200255

    Purpose

    Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    TeTx
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Pnpr-4

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJH4693

    Plasmid

    #200784

    Purpose

    Ptwk-40s EGFP unc-54 3' UTR C.elegans AVA and other neurons expression of EGFP

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    no
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40s

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pJH2673

    Plasmid

    #199691

    Purpose

    Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of FLPe

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    flpE (flpE )
    

    Use

    Tags

    no

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1 long

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pYSD063

    Plasmid

    #212916

    Purpose

    Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBES1-LacOx50-mCh-bla

    Plasmid

    #210260

    Purpose

    Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

    Plasmid

    #199442

    Purpose

    CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    HITI donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-hSyn-ASAP3ER

    Plasmid

    #195358

    Purpose

    ASAP3ER under control of human synapsin promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMET7-GAG-eDHFR

    Plasmid

    #194635

    Purpose

    Mammalian expression of the eDHFR control bait in Virotrap

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    ecDHFR

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUPD2_SulR(CDS)

    Plasmid

    #186423

    Purpose

    provides sulfadiazine resistance (sulI) as a level 0 GoldenBraid part (CDS; B3-B4-B5)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Insert

    SulR
    

    Use

    Synthetic Biology; Cds (part b3-b4-b5), goldenbra…

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFR-mScarletI-antiFRET-P2A-EGFP

    Plasmid

    #191458

    Purpose

    Compare the intensity of EGFP to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    EGFP
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

    Plasmid

    #191459

    Purpose

    Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    mTurquoise2_T203Y
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFR-mTq2-antiFRET-P2A-mCherry

    Plasmid

    #191466

    Purpose

    Compare the intensity of mCherry to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    mCherry
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO_Cep41-STf

    Plasmid

    #182010

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines associated to microtubules

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Cep41-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5/FRT/TO_[Cox8a]x2_STf

    Plasmid

    #182003

    Purpose

    CMV overexpression of SNAP-tag fast in the mitochondrial matrix of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    [Cox8a]x2_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO_CalR_STf_KDEL

    Plasmid

    #182004

    Purpose

    CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    CalR_STf_KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge