We narrowed to 30,996 results for: uros

1. qTAG-N-Puro-moxGFP-TUBA1B

   Plasmid

   #207767

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCW-Cdt1-mCherry-Puro

   Plasmid

   #193763

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   Cdt1-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   WT

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

   Plasmid

   #148859

   Purpose

   Mammalian Expression of HsXRN1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsXRN1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMMLV-mRFP1-P2A-Puro

   Plasmid

   #160228

   Purpose

   Expresses mRFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   mRFP1-P2A-Puro
   

   Use

   Retroviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ki67∆Nterm-mNeonGreen-IRESpuro2

   Plasmid

   #183740

   Purpose

   Expresses Ki67∆Nterm-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain + all Ki-67 repeats (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. neurod1:cre; cmlc2:EGFP

   Plasmid

   #173480

   Purpose

   cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

   Depositor

   John Rawls

   Article

   Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

   Insert

   Cre
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

   Plasmid

   #67975

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiPGK Puro DEST JNKKTRmCerulean3

   Plasmid

   #90232

   Purpose

   Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW-Cdt1ΔPIP-mCherry-Puro

   Plasmid

   #193765

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   Cdt1ΔPIP-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   PIP degron removed (a.a.1-19 removed)

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-TetOne-Puro-MDM4

    Plasmid

    #195140

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQC V5 MCS IRES Puro

    Plasmid

    #110342

    Purpose

    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

    Plasmid

    #112837

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH2 + SH3 (C-term) domains of Grb2

    Promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-puro-miR-9 sponge

    Plasmid

    #25040

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    miR-9 sponge
    

    Use

    Retroviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBbi-RP-CoV2/RBD-puro

    Plasmid

    #161793

    Purpose

    Transposon-based SARS-CoV-2 RBD stable expression vector for protein production

    Depositor

    Jorge Luis Arias-Arias

    Article

    Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

    Insert

    SARS-CoV-2 RBD
    

    Use

    Transposon

    Tags

    6xHis and dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCVpuro-Flag-cMyc T58A

    Plasmid

    #20076

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLC-Flag-CSNK1A1 WT-Puro

    Plasmid

    #123319

    Purpose

    Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-TET3G-T2A-Puro

    Plasmid

    #158067

    Purpose

    Encodes the Tet3G transactivator and puromycin resistance.

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    pCAG-TET3G-T2A-Puro
    

    Use

    AAV

    Tags

    puromycin reistance gene

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid

    #137730

    Purpose

    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro U6 sgRNA CAG

    Plasmid

    #50927

    Purpose

    U6 driven sgRNA negative control

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    negative control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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