We narrowed to 6,263 results for: wus

1. pET-21a_3xNLS_SpCas9_protein_expression

   Plasmid

   #114365

   Purpose

   Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

   Insert

   3xNLS_SpCas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SPLICS NU-MT P2A

   Plasmid

   #210129

   Purpose

   Detect the Nucleus-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

   Insert

   SPLICS NU-MT P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLL5.0 mCherry_K-RasC14

   Plasmid

   #101286

   Purpose

   Lentiviral expression of K-Ras C14

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   K-Ras (KRAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C14

   Promoter

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBoBi-hLAMP2-C-PEN2

   Plasmid

   #183646

   Purpose

   Lentiviral expression of a lysosome-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and LAMP2 (PSENEN, LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH-3_Flag-TET1-S-WT

   Plasmid

   #232942

   Purpose

   Expresses short isoform of TET1 in mammalian cells in its wild-type form

   Depositor

   Jianjun Chen

   Article

   Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

   Insert

   TET1-S-WT (TET1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDH-3_Flag-TET1-S-MUT

   Plasmid

   #232943

   Purpose

   Expresses mutant form of the short isoform of TET1 in mammalian cells

   Depositor

   Jianjun Chen

   Article

   Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

   Insert

   TET1-S-MUT (TET1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   H1652Y and D1654A

   Promoter

   Available Since

   March 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDH-no-Flag-TET1-S-WT

   Plasmid

   #232941

   Purpose

   Expresses short isoform of TET1 in mammalian cells in its wild-type form

   Depositor

   Jianjun Chen

   Article

   Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

   Insert

   TET1-S-WT (TET1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.3-hAP1 1-419

   Plasmid

   #183644

   Purpose

   To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   truncated ATP6AP1 mutant; contains only amino aci…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3-hAP1 420-440

    Plasmid

    #183643

    Purpose

    To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    truncated ATP6AP1 mutant; contains only the trans…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBoBi-hAP1 Δ420-440

    Plasmid

    #183642

    Purpose

    To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    truncated ATP6AP1 mutant lacking the transmembran…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N1-hAIM2

    Plasmid

    #51542

    Purpose

    Express GFP-tagged hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-KIRIN1

    Plasmid

    #107414

    Purpose

    Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HaloTag-bActinCDS-bActinUTR-MS2V5

    Plasmid

    #102718

    Purpose

    Halo-Actin reporter for detection of both mRNA and protein

    Depositor

    Robert Singer

    Article

    Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    HaloTag-bActinCDS-bActin3'UTR-MS2V5
    

    Use

    Lentiviral

    Tags

    HaloTag and MS2V5

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-RLuc8.6-535

    Plasmid

    #87125

    Purpose

    Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pH2B-miRFP680-N1

    Plasmid

    #197229

    Purpose

    Expresses the fusion protein of H2B-miRFP680 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    H2B-miRFP680
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSAM_Cam

    Plasmid

    #91570

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDB4581

    Plasmid

    #171124

    Purpose

    A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    3_sAID
    

    Use

    Other

    Tags

    Expression

    Mutation

    non applicable

    Promoter

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge