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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,398 results for: puc19

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Showing: 1521 - 1540 of 2398 results

  1. pCE27

    Plasmid
    #174405
    Purpose
    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174405-plasmid-map-sequence-id-349710

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  3. pShHELIX_sgRNA_entry (CJT32)

    Plasmid
    #181782
    Purpose
    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181782-plasmid-map-sequence-id-354808

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  19. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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Showing: 1521 - 1540 of 2398 results