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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,028 results for: pET

Showing: 2281 - 2310 of 15028 results

  6. pETM6-3A2-mCherry

    Plasmid
    #73425
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3A2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3A2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73425-plasmid-map-sequence-id-135325

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  8. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available Since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  10. pET11b-MIF Y99A

    Plasmid
    #149440
    Purpose
    Expression of MIF Y99A in E. coli for protein production
    Depositor
    Elias Lolis
    Insert
    MIF (MIF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y99A
    Promoter
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    149440-plasmid-map-sequence-id-311579

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  13. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Use
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available Since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

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  20. pET21-Drp1(deltaIB)

    Plasmid
    #73041
    Purpose
    Bacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Drp1(deltaIB) (Dnm1l Mouse)
    Use
    Tags
    6xHis-tag and PreScission Protease site
    Expression
    Bacterial
    Mutation
    Deleted amino acids 514-602
    Promoter
    T7
    Available Since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73041-plasmid-map-sequence-id-137704

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  24. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Promoter
    Available Since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  29. pET-PesaR-Dlux

    Plasmid
    #47657
    Purpose
    PesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)
    Depositor
    Cynthia Collins
    Article
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-D
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Added an additional esa box upstream of -35 site …
    Promoter
    PesaR-D
    Available Since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2281 - 2310 of 15028 results