We narrowed to 6,962 results for: tet on
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pALS3-Ma-sfGFP-TAG150
Plasmid#212121PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertssfGFP-150TAG
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
rtTA-N144
Plasmid#66810Purpose2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistanceDepositorInsertReverse tetracycline-controlled transactivator
UseLentiviralTagsExpressionMammalianMutationPromoterHuman EF1aAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBL4
Plasmid#50548PurposeExpresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28c S2
Plasmid#171920PurposeExpress RNA-based (p)ppGpp sensorDepositorInsertRNA-based (p)ppGpp sensor
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet S2
Plasmid#171921PurposeExpress RNA-based (p)ppGpp sensor and a NirFPDepositorInsertsRNA-based (p)ppGpp sensor
NirFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-shRNA-ctrl
Plasmid#85741PurposeshRNA ctrlDepositorInsertshRNA ctrl
UseAAVTagsEYFPExpressionMutationPromoterAvailable sinceApril 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCL66-decoder
Plasmid#159453PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AKAP-mtagBFP2-UBC6
Plasmid#105007PurposeMammalian Expression of mtagBFP2DepositorInsertmtagBFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro
Plasmid#211462PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLIDepositorInsertApaLI
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPLV_C1
Plasmid#186415PurposeEndogenous murine RNase inhibitor production for cell-free lysateDepositorInsertMurine RNase Inhibitor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralTagsExpressionMutationPromoterhEF1aAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-Tn5
Plasmid#184286PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmKappa-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG2a-Tn5
Plasmid#184288PurposeAnti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG2a-Tn5
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_a_tubulin_C1
Plasmid#21040DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-ER
Plasmid#36204PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertER-mTurquoise2
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP11_pcDNA3.1(-)
Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRosa26 R1-ccdB-R2 RexNeo PI-SceI
Plasmid#24418DepositorInsert
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TadA*-T7RNAP
Plasmid#167980PurposeExpresses fusion protein TadA*-T7RNAP in E. coliDepositorInsertTadA*-T7RNAP
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
Plasmid#211466PurposeLentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signalDepositorInsertmito-mCherry-GFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PDZD8HA
Plasmid#105005PurposeMammalian Expression of PDZD8DepositorInsertPDZD8 (Pdzd8 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
Inducible tdTomato
Plasmid#183718PurposetdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducerDepositorInsertstdTomato
rTetR
UseLentiviralTagsExpressionMutationPromoterTRE3GV and UbiCAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT for Split-PE (pEH59)
Plasmid#190108PurposeExpresses Marathon-RT (human codon optimization)DepositorInsertMarathon-RT
UseTagsbpNLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL701
Plasmid#140371PurposeExpresses TetR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-TetR-T
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …PromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE-EGFP
Plasmid#89871PurposeEGFP reporter vector for iTango systemDepositorInserttetO-EGFP
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUHD-AID
Plasmid#121066Purposevector expresses AID-fusion protein under TRE promoter and with puromycin resistance selectionDepositorTypeEmpty backboneUseTagsAIDExpressionMammalianMutationPromoterTREAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAraH6HATT
Plasmid#63901PurposeFor expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.DepositorInsertTT Fab
UseTags6xHis and HA tagExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS306-PTDH3-SUMO10-6SIM
Plasmid#190248PurposeExpression SUMO10-SIM6 in yeast cellsDepositorInsert10SUMO-6SIM
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _2
Plasmid#78202PurposeExpression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A D63C T174A T175N D224C C287A)
UseTagsHis6ExpressionBacterialMutationK33A D63C T174A T175N D224C C287APromoterunknownAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pComb3HTT
Plasmid#63900PurposeBackbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.DepositorInsertTT Fab
UsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221AID-T7RNAP
Plasmid#167977PurposeExpresses fusion protein AID-T7RNAP in E. coliDepositorInsertAID-T7RNAP
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6 and SUMOExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB007ns2a
Plasmid#99214PurposeAccessory plasmid for PACE of PylRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
UseTagsExpressionMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PtetAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.717
Plasmid#108517PurposePTet*-YFP Reporter. aTc sensor for use in Marionette strain.DepositorInsertPTet*-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREVTRE-AID
Plasmid#121067PurposeRetrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectioDepositorTypeEmpty backboneUseRetroviralTagsAIDExpressionMammalianMutationPromoterTREAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMrfp
Plasmid#59773PurposeDerived from pCM62; ampR, tetR, PBAD - rfpDepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpltetO1Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFGL1258
Plasmid#116898PurposeTetOFF:GFP:CenpA (centromere marker)DepositorInsertsCenpA promoter region
CenpA
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS011
Plasmid#65464PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherryDepositorInsertMBP-Ntag-mCherry
UseTagsFLAG and MBPExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only
