Addgene

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pcDNA6-N-HA-m-Lmbr1l

Plasmid

#123594

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

limb region 1 like (Lmbr1l Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

Plasmid

#198845

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-R18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

Plasmid

#198841

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

Plasmid

#198840

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-L17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

Plasmid

#198844

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-L18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBT120a17-sg-del

Plasmid

#214347

Purpose

Expresses pIII

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

gIII-xLuxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iAChSnFR

Plasmid

#137955

Purpose

Expresses iAChSnFR under CAG promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1 and AAV9

Insert

iAChSnFR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Lrp6

Plasmid

#123595

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

low density lipoprotein receptor-related protein 6 (Lrp6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-N-3XFLAG-Gsk3b

Plasmid

#123592

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

glycogen synthase kinase 3 beta (Gsk3b Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-N-3XFLAG-Ubxd8

Plasmid

#123599

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Fas associated factor family member 2 (Faf2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iAChSnFR

Plasmid

#137950

Purpose

Expresses iAChSnFR under hSynapsin promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1

Insert

iAChSnFR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynanpsin

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.ZAC.GFAP.iAChSnFR

Plasmid

#137962

Purpose

Expresses iAChSnFR under GFAP promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iAChSnFR-NULL

Plasmid

#137951

Purpose

Expresses iAChSnFR (non-binding) under hSynapsin promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR (non-binding variant)

Use

AAV

Tags

Expression

Mammalian

Mutation

Y140A

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro

Plasmid

#198851

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND1-W86-R17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro

Plasmid

#198861

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro

Plasmid

#198859

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND5-W68-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro

Plasmid

#198857

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND4-W24-R17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro

Plasmid

#198855

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND3-Q26-R19 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro

Plasmid

#198853

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND2-W30-R18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge