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Plasmid#135466PurposeExpression human MSH6_T1219D mutant protein using baculovirusDepositorInserthuman MSH6_T1219D (MSH6 Human)
UseTagsHis-tagExpressionInsectMutationchanged threonine to aspartic acidPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFL-EGFP-3xFlag-DEST
Plasmid#134903PurposeBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tagDepositorTypeEmpty backboneUseTags3x-FlagExpressionInsectMutationPromoterpolyhedronAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFB-Flag-MyoD-E12
Plasmid#25990DepositorInsertMyoD-E12 Heterodimer Fusion (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagExpressionMutationPromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast
Plasmid#213117PurposeExpression of a faster-running MinE mutant in mammalian cellsDepositorInsertMinE(L4E)-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationL4EPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
UseTagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EPromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)
Plasmid#78509PurposeConstruct for expressing CD45R0 (extracellular domain) His10 in Sf9DepositorInsertCD45R0Ex (PTPRC Human)
UseTagsHis10 and SNAPfExpressionInsectMutationPromoterAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC
Plasmid#192406PurposeTo be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.DepositorInsertAct2::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 Y319D
Plasmid#160649PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 PHR Y319D
UseTagsHexa hisExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969PromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
RcSxph E540A
Plasmid#194019PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationE540APromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph E540D
Plasmid#194020PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationE540DPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph E540Q
Plasmid#194021PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationE540QPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only