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pLentiPGK Puro DEST JNKKTRrsGreenF-E

Plasmid

#205764

Purpose

Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

JNK KTR rsGreenF-E

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-CBS(opt)-FLAG

Plasmid

#230088

Purpose

expressing human CBS

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-L2HGDH(opt)-FLAG

Plasmid

#230089

Purpose

expressing human L2HGDH

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

L2HGDH (L2HGDH Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA2

Plasmid

#223522

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2 puro mGSDME gRNA1

Plasmid

#223521

Purpose

Knocking out mGSDME in mouse cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-sgAMPKa2-Cas9-GFP

Plasmid

#208050

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgAMPKa1-Cas9-GFP

Plasmid

#208049

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*Y152*

Plasmid

#191112

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 & Y152 to TAG

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225704

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

pEF1A

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mCherryBSD

Plasmid

#225700

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mCherry

Expression

Mammalian

Mutation

Promoter

pEF1A

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-VP64-2A-mCherryBSD

Plasmid

#225695

Purpose

Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-VP64

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise

Plasmid

#225693

Purpose

Lentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-DNMT3B catalytic domain (DNMT3B Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-EED-2A-mTurquoise

Plasmid

#225692

Purpose

Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-EED (EED Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-HP1a-2A-mTurquoise

Plasmid

#225691

Purpose

Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

HP1a (CBX5 Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-KRAB-2A-mTurquoise

Plasmid

#225690

Purpose

Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-ZNF10 KRAB

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CD80-mApple

Plasmid

#223595

Purpose

Lentiviral expression of human CD80 with mApple in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mApple

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 P652_E653 del

Plasmid

#213700

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

Depositor

Veerle Baekelandt

Article

Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

Insert

human ATP13A2 P652_E653 del (ATP13A2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT, D962N

Promoter

CMV

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SauriCas9-KKH-BlastR

Plasmid

#220972

Purpose

Expresses human codon-optimized SauriCas9-KKH

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9-KKH

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:mApple-he1.1:CFP

Plasmid

#184812

Purpose

Used to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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