We narrowed to 5,818 results for: pcas

1. pFGRNA-LbCpf1-SpCas9

   Plasmid

   #119673

   Purpose

   Empty vector for cloning LbCpf1-SpCas9 fgRNA

   Depositor

   Yongsub Kim

   Article

   Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGRNA-AsCpf1-SpCas9

   Plasmid

   #119674

   Purpose

   Empty vector for cloning AsCpf1-SpCas9 fgRNA

   Depositor

   Yongsub Kim

   Article

   Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-gamma1 (in pCAGGS)

   Plasmid

   #118039

   Purpose

   GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-ePsuACR-mRuby2-ST

   Plasmid

   #109133

   Purpose

   Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ePsuACR-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-eiC++-mRuby2-ST

   Plasmid

   #109132

   Purpose

   Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eiC++-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CsChR-mRuby2-ST

   Plasmid

   #109130

   Purpose

   Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   CsChR-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. zwf-fbp-opcA-

   Plasmid

   #101839

   Purpose

   deletes zwf_fbp_opcA locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   Kanamycin casette (in place of synpcc7942_2333-2335 operon)
   

   Use

   Cloning vector

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCASCADE-udhA-zwf

   Plasmid

   #87153

   Purpose

   udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   udhA-zwf guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. CpcB-Pinus-banksianaPHLS_CpcA

   Plasmid

   #74002

   Purpose

   Expresses the Pinus banksiana PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

   Depositor

   Anastasios Melis

   Article

   Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

   Insert

   PHLS and CmR
   

   Promoter

   cpc

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CpcB-Picea-sitchensisPHLS_CpcA

    Plasmid

    #74001

    Purpose

    Expresses the Picea sitchensis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    PHLS and CmR
    

    Promoter

    cpc

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CpcB-Abies-grandisPHLS_CpcA

    Plasmid

    #74000

    Purpose

    Expresses the Abies grandis PHLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    PHLS and CmR
    

    Promoter

    cpc

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCASCADE-gltA2-udhA

    Plasmid

    #65819

    Purpose

    gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA2-udhA guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCaShs-egghead (zw4)

    Plasmid

    #37400

    Depositor

    Norbert Perrimon

    Article

    Goode et al Development. 1996 Dec;122(12):3863-79.

    Insert

    egghead (egh Fly)
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9(BB)-2A-Puro (PX459) V2.0

    Plasmid

    #62988

    Purpose

    Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9-2A-Puro V2.0
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScRad52-SpCas9 (pXK-1646)

    Plasmid

    #208823

    Purpose

    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

    Depositor

    Kun Xu

    Article

    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

    Type

    Empty backbone

    Use

    CRISPR; Cas9

    Tags

    Rad52

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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