We narrowed to 12,235 results for: Hal
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Plasmid#109658PurposeMuscle specific zebrafish expression of mKate2 fused to rab8bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only
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actc1b-mKate2-rab1ab
Plasmid#109606PurposeMuscle specific zebrafish expression of mKate2 fused to rab1abDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-BTK-mKate2
Plasmid#109501PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.DepositorInsertactc1b-BTK-mKate2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F1
Plasmid#184163PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-FAPP1
Plasmid#109555PurposeMultisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4PDepositorInsertthe PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LF601: pMVP (L1-R5) TBG promoter
Plasmid#121695PurposepMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertTBG promoter (Liver specific)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddtoxA-C
Plasmid#171726PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddtoxA-C
Plasmid#171734PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1397-DddtoxA-N
Plasmid#171735PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1397-DddtoxA-N
Plasmid#171727PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddtoxA-N
Plasmid#171731PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1397-DddtoxA-C
Plasmid#171730PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-EHD1b
Plasmid#109514PurposeMuscle specific zebrafish expression of mKate2 fused to EHD1bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
RP78 pUAST Orco mRNA
Plasmid#53431PurposeExpresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS controlDepositorInsertOrco (Orco )
ExpressionInsectAvailable SinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MBD1_iso1
Plasmid#86263PurposeDonor vector for 3' FLAG tag of human MBD1_iso1DepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168169PurposeExpression of YFP-BeCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
TagsYFPExpressionWormAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-LactC2
Plasmid#109499PurposeMuscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertactc1b-mKate2-LactC2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LC402: pMVP (L3-L2) eGFP + polyA
Plasmid#121763PurposepMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.DepositorInserteGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWL502-PAM
Plasmid#174382PurposePlasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterraneiDepositorInsertPAM-spacer
UseCRISPR; Archaeal expressionExpressionBacterialAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG802: pMVP (L3-L2) V5 epitope tag + polyA
Plasmid#121755PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.DepositorInsertV5 epitope tag + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP472
Plasmid#134997PurposeInterspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertInters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP498
Plasmid#134998PurposeClustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertClust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyExpressionMammalianAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP650
Plasmid#135000PurposeVP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoRDepositorInsertpUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
UseLentiviral and Synthetic BiologyTagsDpnI(aa146-254), V5 tag, and VP-64ExpressionMammalianMutationDpnI truncated to aa146-254Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7a
Plasmid#109655PurposeMuscle specific zebrafish expression of mKate2 fused to rab7aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab7b
Plasmid#109656PurposeMuscle specific zebrafish expression of mKate2 fused to rab7bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST myc MOV10 K530A
Plasmid#51719Purposeplasmid expresses myc-tagged MOV10 K530ADepositorAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab8a
Plasmid#109657PurposeMuscle specific zebrafish expression of mKate2 fused to rab8aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
KW701: pMVP (L5-L4) AID
Plasmid#121723PurposepMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.DepositorInsertAID (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN402: pMVP (R4-R3) Firefly Luciferase
Plasmid#121741PurposepMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertFirefly Luciferase
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
II901: pMVP (L5-L4) FKBP DD
Plasmid#121720PurposepMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.DepositorInsertFKBP Degron Domain (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST myc MOV10 D645N
Plasmid#51720Purposeplasmid expresses myc-tagged MOV10 D645NDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458_MBD1_iso1_2
Plasmid#86304PurposeEncodes gRNA for 3' target of human MBD1_iso1DepositorInsertgRNA against MBD1_iso1 (MBD1 Human)
UseCRISPRAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_GATAD2A_2
Plasmid#86334PurposeEncodes gRNA for 3' target of human GATAD2ADepositorInsertgRNA against GATAD2A (GATAD2A Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_GATAD2A_1
Plasmid#86333PurposeEncodes gRNA for 3' target of human GATAD2ADepositorInsertgRNA against GATAD2A (GATAD2A Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXNtT5R1AtR2NtB2
Plasmid#160888PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T5
ExpressionBacterialPromoterrbcS-T5: T7, chaperones: T11Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT1R1AtR2NtB2
Plasmid#160885PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T1
ExpressionBacterialPromoterrbcS-T1: T7, chaperones: T8Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only