We narrowed to 6,480 results for: poly

1. TBPL1 KO 2

   Plasmid

   #200211

   Purpose

   gRNA for TBPL1 KO

   Depositor

   Sheila Teves

   Article

   Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

   Insert

   TBP1 KO 2 (Tbpl1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. H1.224.luc

   Plasmid

   #193704

   Purpose

   Expresses a truncated PolII H1 promoter (H1.244)

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter of 224 nucleotides (H1.224) + N41 fragment
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. H1.224.M.luc

   Plasmid

   #193705

   Purpose

   Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter (H1.224) with the TATA box mutated
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOP004

   Plasmid

   #185985

   Purpose

   Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator

   Depositor

   Georgi Belogurov

   Article

   Huang et al Methods Enzymol. 2022;675:207-233. doi:

   Insert

   Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOP005

   Plasmid

   #186030

   Purpose

   Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter

   Depositor

   Georgi Belogurov

   Article

   Huang et al Methods Enzymol. 2022;675:207-233. doi:

   Insert

   Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KBB221

   Plasmid

   #185071

   Purpose

   SST::ACT2 "SA236"

   Depositor

   Ken Belanger

   Article

   Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

   Insert

   ACT2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ACT2-SA236

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KBB215

    Plasmid

    #185068

    Purpose

    SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

    Depositor

    Ken Belanger

    Article

    Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

    Insert

    WAK2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CDS_Cas9-NLS

    Plasmid

    #136121

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_AtcoCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLyGo-Bs-4

    Plasmid

    #163142

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLyGo-Ec-4

    Plasmid

    #163130

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLyGo-Ec-1

    Plasmid

    #163127

    Purpose

    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Ubiquitin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLyGo-Bs-3

    Plasmid

    #163141

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLyGo-Bs-2

    Plasmid

    #163140

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLyGo-Ec-3

    Plasmid

    #163129

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLyGo-Ec-5

    Plasmid

    #163131

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX6P1-optimizedNedd4.2F823D

    Plasmid

    #165105

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d F823D

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

    Insert

    Nedd4.2d F823D (NEDD4L Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression; F823D; …

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lenti-Myosin7B-sgRNA

    Plasmid

    #154861

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    MYO7B sgRNA (MYO7B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046774

    Plasmid

    #145574

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046846

    Plasmid

    #145575

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pBMN-AS-FOXA1

    Plasmid

    #139304

    Purpose

    Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

    Depositor

    Claes Wadelius

    Article

    Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

    Insert

    shRNA and amiRNA against FOXA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pY71-tet.I.-1

    Plasmid

    #133539

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pY71-tet.I.25

    Plasmid

    #133538

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pY71-tet.I.20

    Plasmid

    #133537

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pY71-tet.I.14

    Plasmid

    #133535

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pY71-tet.I.13

    Plasmid

    #133534

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pY71-tet.I.12

    Plasmid

    #133533

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pY71-tet.I.11

    Plasmid

    #133532

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pY71-tet.I.10

    Plasmid

    #133531

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pY71-tet.I.9

    Plasmid

    #133530

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pY71-tet.I.7

    Plasmid

    #133529

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pY71-tet.I.6

    Plasmid

    #133528

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pY71-tet.I.5

    Plasmid

    #133527

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pY71-tet.I.3

    Plasmid

    #133525

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pY71-tet.I.2

    Plasmid

    #133524

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pY71-tet.I.4

    Plasmid

    #133526

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pY71-tet.I.1

    Plasmid

    #133523

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET15b-GBdm

    Plasmid

    #129690

    Purpose

    express E. coli GreB E82C/C68S

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S (greB E. coli)
    

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMH-HT_SsCHAD-H29A-R32A-R36A

    Plasmid

    #127786

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32 and Arg36 to Ala

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMH-HT_CtCHAD-R438A-K441A

    Plasmid

    #127791

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg4…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid

    #119865

    Purpose

    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    2x myristoylation

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRPB1–4stop

    Plasmid

    #112824

    Purpose

    pSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codons

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    pSMF2 with repeats 8–10 interrupted with 4 repeat…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. psicheck-2-Tas2r3-mod

    Plasmid

    #48175

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> ACG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    TAS2R3 taste 2 receptor member 3 (TAS2R3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    T/C MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

49. psicheck-2-Phb mod

    Plasmid

    #48163

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Phb (Phb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/T mutation in the ATG of the uORF

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

50. psicheck-2-Acsm2 mod

    Plasmid

    #48159

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Acsm2 (Acsm2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    changed A to T in ATG of uORF

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only