We narrowed to 87,289 results for: lias

1. CROPseq-MPSV-eBFP2

   Plasmid

   #220472

   Purpose

   Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

   Depositor

   Christian Krebs

   Article

   Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDN-D2irTNG4kwh

   Plasmid

   #44722

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   htetR::NLS::EGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter;…

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

   Plasmid

   #183505

   Purpose

   Express GFP and MAN1C1 proteins

   Depositor

   Olivier Guipaud

   Article

   Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

   Inserts

   man1C1 (MAN1C1 Human)
   EGFP
   

   Use

   Lentiviral

   Tags

   Myc-DDK

   Expression

   Mammalian

   Mutation

   Promoter

   MND and hPGK

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYO1025

   Plasmid

   #235705

   Purpose

   Expression of FL human VPS34 and human VPS15-ZZ in mammalian cells

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Inserts

   VPS34 (PIK3C3 Human)
   VPS15 (PIK3R4 Human)
   

   Use

   Tags

   3xTEV-ZZ

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and CMV

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228787

   Purpose

   EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

   Plasmid

   #228788

   Purpose

   EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTyrRS
   EcYtR
   EGFP***
   TEV protease
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CIRTS-YTHDF2-Malat1

   Plasmid

   #216858

   Purpose

   CIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

   Insert

   CIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn1, U6

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIRTS-FTO-Malat1

   Plasmid

   #216860

   Purpose

   CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

   Insert

   CIRTS-FTO-Calm3-U6-Malat1 gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Syn1, U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-CIB1-mRuby-Rab35

   Plasmid

   #194334

   Purpose

   For use in light-induced Rab35 protein inactivation through the interaction with CRY2

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Inserts

   CIB1 (CIB1 Mustard Weed)
   Rab35 (RAB35 Human)
   

   Use

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-SomaGCaMP7

    Plasmid

    #158759

    Purpose

    Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP7
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Syn-SomaGCaMP6f2

    Plasmid

    #158756

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP6f2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-SomaGCaMP6f2

    Plasmid

    #158757

    Purpose

    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Has Service

    AAV8 and AAV9

    Insert

    SomaGCaMP6f2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-SomaGCaMP6f1

    Plasmid

    #158755

    Purpose

    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP6f1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid

    #158758

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    nullCoChR-GCaMP6f-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAtVPZv1.0

    Plasmid

    #233147

    Purpose

    Expresses VDE, PsbS, ZEP from A.thaliana

    Depositor

    Krishna Niyogi

    Article

    Kromdijk et al Science. 2016 Nov 18;354(6314):857-861.

    Inserts

    Resistance to herbicide bialaphos
    Zeaxanthin epoxidase (ABA1 Mustard Weed)
    Photosystem II subunit S (NPQ4 Mustard Weed)
    Violoxanthin de-epoxidase (NPQ1 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    FBA-2, GAPA-1, Rbcs1a, and mas promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-EGFP-V5

    Plasmid

    #200936

    Purpose

    expresses EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lamp1_mStayGold

    Plasmid

    #221902

    Purpose

    Expresses mStayGold fused to Lamp1

    Depositor

    Janelia Integrative Imaging

    Insert

    Lamp1_mStayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-CHMP4B

    Plasmid

    #139013

    Purpose

    Expresses HA-tagged human CHMP4B in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    human CHMP4B
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-UBC-GCAMP6f-PGK-neo

    Plasmid

    #160049

    Purpose

    expression of GCaMP6f in a piggybac plasmid

    Depositor

    Xiaojun Lian

    Insert

    GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-CHMP6

    Plasmid

    #139014

    Purpose

    Expresses HA-tagged human CHMP6 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    human CHMP6
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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