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We narrowed to 2,616 results for: pCas

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Showing: 1541 - 1560 of 2616 results

  1. Td-CBEmax

    Plasmid
    #196600
    Purpose
    Mammalian expression of Td-CBEmax
    Depositor
    Dali Li
    Publication
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196600-plasmid-map-sequence-id-386793

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  2. pX330-COSMC-KO

    Plasmid
    #80008
    Purpose
    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.
    Depositor
    Sriram Neelamegham
    Publication
    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COSMC (C1GALT1C1 Human)
    Use
    CRISPR
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    80008-plasmid-map-sequence-id-153264

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  3. pAK005

    Plasmid
    #233642
    Purpose
    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.
    Depositor
    Zak Swartz
    Publication
    Clarke et al Development. 2024 Oct 15;151(20):dev2028
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9(1.1) with c-terminal VitelloTagB
    Tags
    6-His
    Expression
    Mammalian
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    233642-plasmid-map-sequence-id-469019

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  5. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid
    #139086
    Purpose
    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139086-plasmid-map-sequence-id-273882

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  6. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101725-plasmid-map-sequence-id-199888

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  7. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  9. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Publication
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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  12. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid
    #219964
    Purpose
    Express human BCR-ABL1-P190 in mammalian cells
    Depositor
    Mohamed Fareh
    Insert
    BCR-ABL1-P190 (ABL1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219964-plasmid-map-sequence-id-442484

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  13. pCMV_T7-PE2-Nuclease

    Plasmid
    #171997
    Purpose
    Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV_T7-Cas9-RT
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171997-plasmid-map-sequence-id-338159

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  14. pAblo

    Plasmid
    #207528
    Purpose
    Conditionally-replicating in Pseudomonas plasmid for adenine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sg
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→ ABE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207528-plasmid-map-sequence-id-410861

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  20. PP7 guide

    Plasmid
    #86168
    Purpose
    Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells
    Depositor
    Amit Choudhary
    Publication
    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7 guide
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86168-plasmid-map-sequence-id-171565

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Showing: 1541 - 1560 of 2616 results