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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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LZRS-BRaf-ERTm

Plasmid

#24949

Depositor

Paul Khavari

Article

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

BRaf-ER (Esr1, BRAF Mouse, Human)

Use

Retroviral

Tags

Myc

Mutation

V600E in BRAF

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA_DENV2-NS2B3_V5

Plasmid

#115906

Purpose

Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

Depositor

Alan Rothman

Article

Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

Insert

DENV2 NS2B3 protease

Tags

V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-PHIP

Plasmid

#65389

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PHIP (PHIP Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pWPI-E2F1

Plasmid

#114296

Purpose

Constitutive expression of the E2F1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F1 (E2F1 Human)

Use

Lentiviral

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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Cas9/pTREX-n

Plasmid

#68708

Purpose

Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .

Use

CRISPR

Tags

Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

Mutation

C4239T mutation that eliminates a BamHI restricti…

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMRXIP VPS16-GFP

Plasmid

#67023

Purpose

Human VPS16 ORF was inserted into pMRXIP GFP-N vector

Depositor

Noboru Mizushima

Article

Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

Insert

Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only
pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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LV-hGFAP-GFP

Plasmid

#183906

Purpose

Expression of GFP under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

Insert

GFP (GFAP Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1

Plasmid

#114022

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-tagRFP-Mad2

Plasmid

#114021

Purpose

Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)

Use

Lentiviral

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pME-cas9-2A-mCherry

Plasmid

#107592

Purpose

An entry vector with cas9 -2A-mCherry

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

cas9-2A-mCherry

Use

CRISPR

Tags

2A-mCherry

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pB-actin E6 E7

Plasmid

#13712

Depositor

Karl Munger

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV 16 E6 E7 (E6, E7 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-RTN3L

Plasmid

#128264

Purpose

Expresses RTN3L in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

RTN3 (RTN3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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TRX1 His C32S C35S

Plasmid

#21286

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

His

Expression

Mammalian

Mutation

C32S C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pMT1002

Plasmid

#8621

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

barnase, barstar

Tags

as pMT416

Expression

Bacterial

Mutation

CI157 lambda repressor gene has two HindIII sites…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pGV13 (CK2alpha K68M, CK2beta)

Plasmid

#27094

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha K68M (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA on CK2alpha and Myc on CK2beta

Expression

Mammalian

Mutation

K68M on CK2alpha

Promoter

bidirectional tet-responsive promoter and bidirec…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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p3036 GST-HPV-16 E6

Plasmid

#10849

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

HPV16 E6 (E6 Human papillomavirus)

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only