We narrowed to 4,132 results for: pCMV mammalian

1. G1333 DddAtox-N–dSpCas9

   Plasmid

   #157833

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SaCas9 TadCBEa

   Plasmid

   #193844

   Purpose

   Express TadCBEa (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   SaCas9 TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. eNme2-C TadCBEa

   Plasmid

   #193840

   Purpose

   Express TadCBEa (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   eNme2-C TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SaBE3-Gam

   Plasmid

   #100810

   Purpose

   Expresses SaBE3-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   SaBE3-Gam
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. JPF0520

   Plasmid

   #124011

   Purpose

   Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pCMV-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK6_003

   Plasmid

   #123857

   Purpose

   Encodes pCMV-Blasticidin resistance cassette as a type 6 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   CMV-Blast-bgPA
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

   Plasmid

   #169664

   Purpose

   Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PhCMV / RPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH19-Tier1-PhCMV-p300core

   Plasmid

   #169572

   Purpose

   Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P300core (EP300 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

   Plasmid

   #169743

   Purpose

   CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ARB344

    Plasmid

    #124027

    Purpose

    Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    L1-B1_CMV-NES::iRFP713-rglPA_R2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ARB345

    Plasmid

    #124028

    Purpose

    Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    L1-B1_CMV-NES::tagBFP-rglPA_R2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ARB346

    Plasmid

    #124029

    Purpose

    Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    L1-B1_CMV-NES::mAzamiGreen-rglPA_R2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ARB347

    Plasmid

    #124030

    Purpose

    Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    L1-B1_CMV-NES::mRuby2-rglPA_R2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. JPF0513

    Plasmid

    #124004

    Purpose

    Encodes pCMV-A driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-A-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JPF0514

    Plasmid

    #124005

    Purpose

    Encodes pCMV-B driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-B-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JPF0515

    Plasmid

    #124006

    Purpose

    Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-C-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JPF0516

    Plasmid

    #124007

    Purpose

    Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-D-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JPF0517

    Plasmid

    #124008

    Purpose

    Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-E-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JPF0518

    Plasmid

    #124009

    Purpose

    Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-F-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JPF0519

    Plasmid

    #124010

    Purpose

    Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-G-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MTK6_009

    Plasmid

    #123863

    Purpose

    Encodes pCMV-Hygromycin resistance cassette as a type 6 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    CMV-Hygro-bgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ABE8e

    Plasmid

    #138489

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ND6-L1397-N_UGI-NES_right

    Plasmid

    #187846

    Purpose

    expresses the right-side half ND6-UGI-NES in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–NES–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ND6-L1397-N_UGI-NES_left

    Plasmid

    #187845

    Purpose

    expresses the left-side half ND6-UGI-NES in mammalian cells

    Depositor

    Chengqi Yi

    Article

    Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–hivNES–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SaABE8e

    Plasmid

    #138500

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid

    #179682

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Calnexin-HT8-mGold

    Plasmid

    #183989

    Purpose

    Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Tags

    HaloTag8 (C61S, C262S), mGold

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pC0079 RESCUE-S

    Plasmid

    #130662

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mNeonGreen-α-synuclein

    Plasmid

    #205713

    Purpose

    Mammalian expression of mNeonGreen-alpha-synuclein

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    alpha-synuclein (SNCA Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. m6A-Tracer-NES

    Plasmid

    #159607

    Purpose

    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    m6A-Tracer-NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LbABE8e

    Plasmid

    #138504

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dLbCas12a
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p3xFLAG-MKL1

    Plasmid

    #11978

    Purpose

    For mammalian expression of human MKL1 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N579S T716A

    Promoter

    CMV

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. DD-DamV133A-tdTomato-LMNB1

    Plasmid

    #159604

    Purpose

    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    DD-DamV133A-tdTomato-LMNB1
    

    Expression

    Mammalian

    Mutation

    Dam V133A mutation

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Calnexin-HT-mGold

    Plasmid

    #183987

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Tags

    HaloTag, mGold

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ND4.2-Left TALE-G1397-N-DddA11

    Plasmid

    #179681

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    S1330I A1341V N1342S E1370K T1380I

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Pak3

    Plasmid

    #99510

    Purpose

    to express Pak3 in mammalian system

    Depositor

    Dong-Yan Jin

    Article

    Chan et al Retrovirology. 2013 Apr 26;10:47. doi: 1

    Insert

    Pak3 (Pak3 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid

    #221997

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge