We narrowed to 415 results for: ipsc

1. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTNT-B18R

   Plasmid

   #58978

   Purpose

   expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   B18R
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Sp6

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-P-MCS

   Plasmid

   #80488

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTALdNC-AAVS1_T1

   Plasmid

   #80495

   Purpose

   AAVS1 TALEN expression vector (Left)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Left)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTALdNC-AAVS1_T2

   Plasmid

   #80496

   Purpose

   AAVS1 TALEN expression vector (Right)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Right)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-Nst-MCS

   Plasmid

   #80487

   Purpose

   donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPV-EF1a-EGxxFP-iP-A

   Plasmid

   #204624

   Purpose

   Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX-EGFP-g1

    Plasmid

    #107273

    Purpose

    eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP sgRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSicoR-ADAM29i1

    Plasmid

    #59606

    Purpose

    Expression of shRNA against human ADAM29

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pX-APRT-sg2

    Plasmid

    #107274

    Purpose

    APRT sgRNA-2 and Cas9 expression vector

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    APRT sgRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPV-C1-crRNA-cloning-EF1a-BA

    Plasmid

    #204623

    Purpose

    piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TAB-mOvol1

    Plasmid

    #120360

    Purpose

    piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSicoR-CENPBi2

    Plasmid

    #59619

    Purpose

    Expression of shRNA against human CENPB

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    CENPB (CENPB Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pSicoR-ADAM29i2

    Plasmid

    #59607

    Purpose

    Expression of shRNA against human ADAM29

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. AAVS1-Bxb1-LP-v2-TC

    Plasmid

    #194326

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXAT2

    Plasmid

    #80494

    Purpose

    AAVS1 sgRNA expression vector

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 sgRNA-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T7-VEE-IRES-Puro

    Plasmid

    #58970

    Purpose

    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Type

    Empty backbone

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. CLYBL-Bxb1-LP-v2-TC

    Plasmid

    #194327

    Purpose

    CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge