1,878 results

1. pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

   Plasmid

   #159817

   Purpose

   Positive control for germline GFP expression from simple array in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

   Plasmid

   #159808

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

   Plasmid

   #159897

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | gfp | cbr-tbb-2 UTR
   

   Expression

   Worm

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdestDD04Neo

   Plasmid

   #26386

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial and Worm

   Available Since

   Oct. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ212 - pDESTR4-R3(cxTi10816, IV)

   Plasmid

   #34864

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   cxTi10816 targeting
   

   Use

   Worm targeting

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ421 - Pmyo-2::GFP::H2B (pharynx)

   Plasmid

   #34876

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   Pmyo-2 GFP H2B tbb-2 UTR
   

   Use

   Worm targeting

   Mutation

   S65C

   Promoter

   myo-2

   Available Since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. coel::GFP

   Plasmid

   #8937

   Depositor

   Piali Sengupta

   Article

   Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

   Insert

   unc-122 promoter (unc-122 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

   Plasmid

   #193048

   Purpose

   Heatshock expressed Cas9

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   hsp16.41p

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJA58

   Plasmid

   #59933

   Purpose

   gRNA for cleavage at dpy-10(cn64) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   dpy-10(cn64) gRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159857

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

    Plasmid

    #193049

    Purpose

    Encodes G-418 resistance

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    prsp-27::NeoR::unc-54 3’ UTR
    

    Expression

    Worm

    Promoter

    prsp-27

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNM3823

    Plasmid

    #154123

    Purpose

    mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::tetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid

    #107939

    Purpose

    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    mCherry(dpiRNA)::ANI-1(AHPH)
    

    Expression

    Worm

    Promoter

    pie-1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNM3755

    Plasmid

    #154116

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::act-4 3'
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ2369 - intron GG1 - 250bp

    Plasmid

    #159877

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG1 - 250bp
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEM320 - sgRNA mosTI unc-119 array insertion

    Plasmid

    #159824

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 array insertion
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCFJ1137

    Plasmid

    #84827

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Expression

    Bacterial and Worm

    Promoter

    Peft-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNMSB84

    Plasmid

    #199309

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJW2171

    Plasmid

    #163095

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF11-wElectra1

    Plasmid

    #184932

    Purpose

    Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1
    

    Expression

    Worm

    Promoter

    tag-168

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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