1,926 results

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pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

Plasmid

#159836

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::GCaMP6s

Plasmid

#124351

Purpose

Nuclear Localized Pan-Neuronal GCaMP6s

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

rab-3, GCaMP6s (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJF5

Plasmid

#139031

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

gfp (no STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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KG#121

Plasmid

#110879

Purpose

Expresses the C. elegans unc-31 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

rab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

Missing first 408bp

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEY11

Plasmid

#191017

Purpose

Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

Ultrapanneuronal

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Pflp-18::NGL-1::spGFP11

Plasmid

#65828

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP11

Expression

Worm

Mutation

N142S

Promoter

Pflp-18

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159813

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTG613

Plasmid

#237388

Purpose

mStayGold::AID^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold::AID^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP

Plasmid

#232608

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pJH23

Plasmid

#159314

Purpose

Punc-25 GFP::SYD-2

Depositor

Mei Zhen

Article

Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

Insert

syd-2 (syd-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Punc-25

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJR022

Plasmid

#198820

Purpose

C. elegans optimized version of mScarlet

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::wrmScarlet::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCCM935

Plasmid

#58202

Purpose

Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

unc-22 sgRNA

Expression

Worm

Promoter

U6

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pDD362

Plasmid

#137023

Purpose

SapTrap HaloTag-GLO donor

Depositor

Daniel Dickinson, Bob Goldstein

Insert

C. elegans GLO-optimized Halo Tag

Use

CRISPR; Saptrap donor for cloning repair templat…

Tags

C. elegans GLO-optimized Halo Tag

Expression

Worm

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHW581

Plasmid

#198818

Purpose

In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR

Expression

Worm

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJJS002

Plasmid

#188325

Purpose

PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::GFP::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

Plasmid

#154840

Purpose

Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

HYGR∆ encodes aa 1-226

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGLOW79

Plasmid

#177339

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-3

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEY88

Plasmid

#191081

Purpose

ggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-1

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-hif-1-sgRNA-A

Plasmid

#177784

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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