1. pX330-U6-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #42230

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. ULK1 KO sgRNA

   Plasmid

   #207561

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CCCGCCTGCGCCATGGAGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ATM N-terminal sgRNA

   Plasmid

   #207089

   Purpose

   pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ATCATTAAGTACTAGACTCA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. bbCas9pluspAAA

   Plasmid

   #82581

   Purpose

   The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

   Depositor

   Ivo Huijbers

   Article

   Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CRISPRmTmG2

   Plasmid

   #69992

   Purpose

   The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   gRNA that targets near LoxP sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCUX1.1.0-gDNA

   Plasmid

   #112434

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1

   Depositor

   Kevin White

   Insert

   CUX1 (CUX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RNF168 C-terminal sgRNA

   Plasmid

   #207100

   Purpose

   pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GAGATGCACAAAGTAAGGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RIF C-terminal sgRNA

   Plasmid

   #207096

   Purpose

   pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   AATACTAAATAGAATTTTCA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. REV7 C-terminal sgRNA

   Plasmid

   #207095

   Purpose

   pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GCTCATAAAGGCAGCTGAGG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Halo XLF sgRNA

   Plasmid

   #207605

   Purpose

   sgRNA for the insert of the HaloTag at the endogenous loci of XLF

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   GTTCTTCCATctgcaaaaaa
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HaloXRCC4 sgRNA

    Plasmid

    #207606

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    TACTGGGTTCAGAAACAAGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HaloKu70 sgRNA

    Plasmid

    #207583

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    GAGCAGTAGCCAACATGTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SHLD3 N-terminal sgRNA

    Plasmid

    #207094

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTACTGCAGAATGACTACAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NBS1 C-terminal sgRNA

    Plasmid

    #207092

    Purpose

    pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TAAAAAGGAGAAGATAACTG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RNF169 C-terminal sgRNA

    Plasmid

    #207099

    Purpose

    pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ACACTTCATTAGGTGCTACT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SHLD1 N-terminal sgRNA

    Plasmid

    #207101

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATGGCAGGACTATGGCAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ATM C-terminal sgRNA

    Plasmid

    #207097

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTCTAAAGGCTGAATGAAA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SHLD3 KO sgRNA #1

    Plasmid

    #207105

    Purpose

    pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CGCTATCAAGATTTATACCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MDC1 KO sgRNA

    Plasmid

    #207103

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGTGTAACGTGGAGCCAGTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATG13 sgRNA

    Plasmid

    #207558

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    ggaaactgatctcaattccc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SHLD2 N-terminal sgRNA

    Plasmid

    #207098

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTTTATCAGAAATCATGAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HaloTag KO sgRNA

    Plasmid

    #207102

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTCGATGTTGGTCCGCGCGA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MDC1 N-terminal sgRNA

    Plasmid

    #207090

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTATCCTTCCCAGATCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SHLD3 KO sgRNA #2

    Plasmid

    #207106

    Purpose

    pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CTGAAGGAACAGACTAATTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 53BP1 KO sgRNA

    Plasmid

    #207104

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGATTCTCAGCCTGAAAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMAZ.1.0-gDNA

    Plasmid

    #112455

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MAZ

    Depositor

    Kevin White

    Insert

    MAZ (MAZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTHRB.1.0-gDNA

    Plasmid

    #112429

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor THRB

    Depositor

    Kevin White

    Insert

    THRB (THRB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CRISPR_HLA_class_I_2

    Plasmid

    #164987

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CRISPR_HLA_class_I_1

    Plasmid

    #164986

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge