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We narrowed to 457 results for: Si

Showing: 351 - 400 of 457 results

  2. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

    Plasmid
    #202090
    Purpose
    Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations
    Depositor
    Elizabeth Heller
    Article
    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8
    Insert
    dCas9-Set2(R195G, C201A)
    Use
    Tags
    3x FLAG and mCherry
    Expression
    Mammalian
    Mutation
    R195G and C201A abolish catalytic activity
    Promoter
    human Synapsin
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    202090-plasmid-map-sequence-id-402835

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  3. mitoTALECD for_atp1_1333NC

    Plasmid
    #186202
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333C of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186202-plasmid-map-sequence-id-380568

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  4. mitoTALECD for_atp1_1397NC

    Plasmid
    #186204
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis RPS5A
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186204-plasmid-map-sequence-id-368339

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  5. mitoTALECD for_atp1_1333CN

    Plasmid
    #186201
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333B of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186201-plasmid-map-sequence-id-368308

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  8. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  16. TFORF2716

    Plasmid
    #144152
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SLC2A4RG (SLC2A4RG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144152-plasmid-map-sequence-id-396220

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  17. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  20. mitoTALECD for_-20G_1333CN

    Plasmid
    #186200
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186200-plasmid-map-sequence-id-368306

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  21. mitoTALECD for_ -13G_1397CN

    Plasmid
    #186199
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186199-plasmid-map-sequence-id-368290

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  22. mitoTALECD for_ -6G_1333CN

    Plasmid
    #186198
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186198-plasmid-map-sequence-id-368288

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  29. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid
    #194190
    Purpose
    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    5 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194190-plasmid-map-sequence-id-381947

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  30. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid
    #194193
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194193-plasmid-map-sequence-id-381931

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  43. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid
    #194195
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194195-plasmid-map-sequence-id-381917

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  44. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid
    #194196
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194196-plasmid-map-sequence-id-381925

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  45. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid
    #194197
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 449)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194197-plasmid-map-sequence-id-381946

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  46. pGL3-pSMANTIS_SMAD-FOS_Mutant

    Plasmid
    #194188
    Purpose
    Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated SMAD/FOS Site, Region 3-15nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194188-plasmid-map-sequence-id-381782

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  47. pGL3-pSMANTIS_STAT1-2_Mutant

    Plasmid
    #194189
    Purpose
    Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated STAT1/2 site, Region 653-673nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194189-plasmid-map-sequence-id-381784

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  48. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid
    #194191
    Purpose
    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194191-plasmid-map-sequence-id-381776

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  49. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid
    #194192
    Purpose
    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    3 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194192-plasmid-map-sequence-id-381933

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  50. pGL3-pSMANTIS_1kb_KLF2mut 548

    Plasmid
    #194194
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 548)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194194-plasmid-map-sequence-id-381930

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Showing: 351 - 400 of 457 results