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Showing: 351 - 400 of 599 results
  1. EC71167

    Plasmid
    #154066
    Purpose
    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP
    Depositor
    Insert
    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    All BsaI, Esp3I and BPiI restriction sites were r…
    Promoter
    Zea mays Ubiquitin Promoter
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pJH4508

    Plasmid
    #200256
    Purpose
    Pflp-18 LoxP EBFP LoxP TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of TeTx RFP
    Depositor
    Insert
    TeTx
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    Promoter
    Pflp-18
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pHR-LS

    Plasmid
    #205438
    Purpose
    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes
    Depositor
    Inserts
    Gaussia luciferase gene
    spectinomycin resistance gene
    Use
    Cre/Lox and Luciferase; Chlamydomonas expression
    Tags
    Expression
    Mutation
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid
    #170186
    Purpose
    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing
    Depositor
    Insert
    CreERT2-T2A-eBFP2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pDsRed-attP

    Plasmid
    #51019
    Purpose
    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and None
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pRosa26-GT

    Plasmid
    #40025
    Depositor
    Inserts
    GFP
    beta-globin intron
    Neo
    tdT-3Myc
    diphteria toxin A
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta globin promoter and CMV enhance…
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
  7. pD/Paro-IFN

    Plasmid
    #205439
    Purpose
    A donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genes
    Depositor
    Inserts
    dog interferon α-4 gene
    paromomycin resistance gene
    Use
    Cre/Lox; Chlamydomonas expression
    Tags
    Expression
    Mutation
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pTol2-CreLite

    Plasmid
    #131783
    Purpose
    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.
    Depositor
    Insert
    PhyBCreC, PIF6CreN, and mTagBFP2
    Use
    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Ubi
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pJH4562

    Plasmid
    #200780
    Purpose
    Pflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFP
    Depositor
    Insert
    GtAR2
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    Promoter
    Pflp-18
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pJH4609

    Plasmid
    #200781
    Purpose
    Ppdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and other neurons expression of GtACR2 RFP
    Depositor
    Insert
    GtACR2
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    Promoter
    Ppdf-1
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pJH4140

    Plasmid
    #191479
    Purpose
    Pacr-2s-loxP::EBFP::loxP::Chrimson::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP
    Depositor
    Insert
    Chrimson
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pacr-2s
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pJH4235

    Plasmid
    #191534
    Purpose
    Psra-11-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVB/others neuron expression of GtACR2 RFP
    Depositor
    Insert
    GtACR2
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Psra-11
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pJH4253

    Plasmid
    #191539
    Purpose
    Prig-3-LoxP::EBFP::LoxP::Chrimson::wCherry unc-54 3' UTR C.elegans AVA neuron expression of Chrimson RFP
    Depositor
    Insert
    Chrimson
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Prig-3
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pJH4211

    Plasmid
    #191532
    Purpose
    Pacr-2s-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans A/B MN expression of GtACR2 RFP
    Depositor
    Insert
    GtACR2
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pacr-2s
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pJH4217

    Plasmid
    #191533
    Purpose
    Prig-3-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVA neuron expression of GtACR2 RFP
    Depositor
    Insert
    GtACR2
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Prig-3
    Available since
    Nov. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. MTK1_015

    Plasmid
    #123678
    Purpose
    Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system
    Depositor
    Insert
    Connector LS-2xHS4-LoxP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pAAV-CreLite

    Plasmid
    #131785
    Purpose
    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter
    Depositor
    Insert
    PhyBCreC-P2A-PIF6CreN
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  18. pMADM-alpha

    Plasmid
    #36890
    Depositor
    Inserts
    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, deleted…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
  19. pTol2-PcyA-IRES-HO1

    Plasmid
    #131787
    Purpose
    Tol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.
    Depositor
    Insert
    PcyA and HO1
    Use
    Synthetic Biology; Zebrafish tol2 transposon
    Tags
    MTS (Mitochondrial Targeting Signal from subunit …
    Expression
    Mammalian
    Mutation
    Promoter
    hsp70
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. pJH4316

    Plasmid
    #200171
    Purpose
    Pflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFP
    Depositor
    Insert
    twk-40 (twk-40 Nematode)
    Use
    Tags
    GFP
    Expression
    Worm
    Mutation
    Promoter
    Pflp-18
    Available since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  21. pCALNL-tdTomato

    Plasmid
    #194205
    Purpose
    Cre/LoxP-dependent expression of a tdTomato. Expression of Cre excises a STOP codon before tdTomato, facilitating fluorescence expression of tdTomato.
    Depositor
    Insert
    tdTomato
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  22. pBT272_(pRosa26-GTET)

    Plasmid
    #36882
    Depositor
    Inserts
    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    diphteria toxin A
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, inserti…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
  23. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid
    #229810
    Purpose
    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Insert
    loxp-AmCyan-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  24. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid
    #229814
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Insert
    loxp-EGFP-stop-lox
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid
    #229816
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.
    Depositor
    Insert
    loxp-EGFP-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid
    #229841
    Purpose
    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre
    Depositor
    Insert
    loxp-H2B-mCerulean-2xStop-loxP
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pBCSK_LEU2_II

    Plasmid
    #109061
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.
    Depositor
    Insert
    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pBCSK_LEU2_I

    Plasmid
    #109060
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes
    Depositor
    Insert
    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  30. pCambia-loxKan

    Plasmid
    #199590
    Purpose
    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility
    Depositor
    Insert
    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    Use
    Cre/Lox
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Nos
    Available since
    July 5, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  31. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Insert
    HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 351 - 400 of 599 results