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pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

Plasmid

#66779

Purpose

The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

Depositor

Thomas Oertner

Article

Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

Inserts

hbeRhGC
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

Plasmid

#172105

Purpose

Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)-Gαs (GNAS Human)

Use

Tags

Expression

Mammalian

Mutation

Gαs: C3S

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gαs-LgB113

Plasmid

#134362

Purpose

Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gαs-LgB113 (GNAS Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1.Gα11-LgB97

Plasmid

#134361

Purpose

Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

Gα11-LgB97 (GNA11 Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hTyro

Plasmid

#225998

Purpose

Expresses a chimeric construct having N-terminal human EGFR and C-terminal human Tyro3

Depositor

Sergei Kotenko, Raymond Birge

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Tyro3 C-Terminal Fusion (EGFR, TYRO3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hMer

Plasmid

#225996

Purpose

Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

Depositor

Sergei Kotenko, Raymond Birge

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hAxl

Plasmid

#225997

Purpose

Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

Depositor

Sergei Kotenko, Raymond Birge

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-YFP II (pMIY II)

Plasmid

#52108

Purpose

a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-EYFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-DsRed FP (pMIDsRed II)

Plasmid

#52110

Purpose

a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-DsRed

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-CFP II (pMIC II)

Plasmid

#52109

Purpose

a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-ECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 DPC4-HA

Plasmid

#14038

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (SMAD4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP II (pMIG II)

Plasmid

#52107

Purpose

a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-DPC4

Plasmid

#14039

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-PHLPP2

Plasmid

#22403

Depositor

Alexandra Newton

Article

Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

Insert

PHLPP2 (PHLPP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV5 DPC4 (1-514)

Plasmid

#14040

Depositor

Joan Massague

Article

Lagna et al Nature. 1996 Oct 31. 383(6603):832-6.

Insert

DPC4 (1-514) (SMAD4 Human)

Use

Tags

Expression

Mammalian

Mutation

R515 was mutated into a stop codon. AGA to TGA. D…

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3-STAT3-Y705F

Plasmid

#74434

Purpose

Expression of STAT3-Y705F

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Use

Tags

Flag

Expression

Mammalian

Mutation

Mutations E16K, Y705F

Promoter

CMV

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-STAT3-S727D

Plasmid

#73364

Purpose

Expression of STAT3-S727D

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Use

Tags

FLAG tagged

Expression

Mammalian

Mutation

E16K S727D

Promoter

CMV

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTriExEKAR2G2

Plasmid

#40175

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Use

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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