We narrowed to 2,461 results for: T2A

1. PB-CRX-T2A-NeuroD1

   Plasmid

   #194607

   Purpose

   Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

   Depositor

   Haruhisa Inoue

   Article

   Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

   Insert

   CRX-T2A-NeuroD1
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_T2A_mCherry sg1-sg2

   Plasmid

   #192479

   Purpose

   All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti:FlipGFP-T2A-mCherry

   Plasmid

   #223198

   Purpose

   Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

   Depositor

   Yeh-Hsing Lao

   Article

   Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

   Insert

   FlipGFP-T2A-mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

   Plasmid

   #169325

   Purpose

   Expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

   Plasmid

   #89347

   Purpose

   2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

   Depositor

   Ron Vale

   Article

   Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

   Insert

   Jurkat TCRalpha/beta alleles and CD3e and CD3z
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-T2A-HygR

   Plasmid

   #118153

   Purpose

   SpCas9 expression vector

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   hSpCas9-T2A-HygR
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-T2A-moxMaple3

   Plasmid

   #120875

   Purpose

   Mammalian expression of mCherry and moxMaple3

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Inserts

   mCherry
   moxMaple3
   

   Expression

   Mammalian

   Mutation

   from mMaple3, moxMaple3 was engineered by replaci…

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUG-T2A-cre

   Plasmid

   #66691

   Purpose

   Recombine floxed alleles in any cell

   Depositor

   Bryan Luikart

   Article

   Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

   Insert

   GFP-T2A-Cre
   

   Use

   Lentiviral

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 N-T2A-M-IRES-E

    Plasmid

    #231903

    Purpose

    For the production of SARS-CoV-2 virus-like particles (VLPs) in the '3-plasmid' system

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    nucleocapsid protein-T2A-membrane protein-IRES-envelope protein (N, M, E SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    R203M in N protein

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. CA-Stat3-t2A-mCherry

    Plasmid

    #127540

    Purpose

    Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Stat3 (Stat3 Mouse)
    

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders …

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh; U6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-GFP-MT2A

    Plasmid

    #11613

    Depositor

    Steven Johnson

    Insert

    metallothionein 2A (MT2A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSXneo 270(T2AG3)

    Plasmid

    #12403

    Depositor

    Titia de Lange

    Article

    Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

    Insert

    270(T2AG3)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2C-EGFP-t2a-mCD4

    Plasmid

    #216213

    Purpose

    Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

    Depositor

    Michelle Percharde

    Article

    Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

    Insert

    CD4 (Cd4 Mouse)
    

    Tags

    GFP-T2A

    Expression

    Mammalian

    Mutation

    CD4 transmembrane domain only

    Promoter

    MERVL

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSXneo 135(T2AG3)

    Plasmid

    #12402

    Depositor

    Titia de Lange

    Article

    Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

    Insert

    135(T2AG3)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCas9-T2A-BFP

    Plasmid

    #78547

    Purpose

    Cas9 protein with BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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