Addgene

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pPD385 VPR-ZF43(RAAA)

Plasmid

#138824

Purpose

Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (AAA)

Plasmid

#24241

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AAA) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 44 to Alanine changed Threonine 5…

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-human-pro-IL-18_LES33AAA

Plasmid

#214321

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

LES33AAA

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM11-6xHis-TEV-hORF1p_StammerAAA

Plasmid

#202587

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

ORF1p

Use

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Mutation

Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH

Plasmid

#148939

Purpose

Insect Expression of DmGIGYF-WFFtoAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-WFFtoAAA (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-CLL78AAA_Z

Plasmid

#148189

Purpose

Bacterial Expression of DmThor-CLL78AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-CLL78AAA (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-NFCVYRHAtoAAYYAAAF_X

Plasmid

#147978

Purpose

Insect Expression of DmCaf40-NFCVYRHAtoAAYAAAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYRHAtoAAYAAAF (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-FWK41AAA_T

Plasmid

#147603

Purpose

Insect Expression of Dm4E-T-FKW41AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-FKW41AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T-FWK41AAA_T

Plasmid

#147611

Purpose

Insect Expression of Dm4E-T-FWK41AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-FWK41AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-NRE90AAA_T

Plasmid

#147577

Purpose

Insect Expression of Dm4E-T-NRE90AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-NRE90AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T-NRE90AAA_T

Plasmid

#147582

Purpose

Insect Expression of Dm4E-T-NRE90AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-NRE90AAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148410

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148429

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTS2415-Tier1-PPGK-1xEAAAK

Plasmid

#169482

Purpose

Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven EAAAK-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPD387 VPR-ZF43(AAAR)

Plasmid

#138825

Purpose

Constitutive expression of VPR-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD386 VP64-ZF43(AAAR)

Plasmid

#138821

Purpose

Constitutive expression of VP64-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD384 VP64-ZF43(RAAA)

Plasmid

#138820

Purpose

Constitutive expression of VP64-ZF43(RAAA) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-ZF43(RAAA)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R15A, R43A, R71A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD112 VP16-ZF43(AAAR)

Plasmid

#138817

Purpose

Constitutive expression of VP16-ZF43(AAAR) under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF43(AAAR)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

R6A, R15A, R43A

Promoter

CMV

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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