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Plasmid#167624PurposeExpress TonB C18G, N208C.DepositorInserttonB C18G, N208C
UseTagsExpressionBacterialMutationtonB with C18G, N208C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1867
Plasmid#167620PurposeExpress TonB C18G, R204C.DepositorInserttonB C18G, R204C
UseTagsExpressionBacterialMutationtonB with C18G, R204C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1676
Plasmid#167618PurposeExpress TonB C18G, M201C.DepositorInserttonB C18G, M201C
UseTagsExpressionBacterialMutationtonB with C18G, M201C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1427
Plasmid#167616PurposeExpress TonB C18G, Q160C.DepositorInserttonB C18G, Q160C
UseTagsExpressionBacterialMutationtonB with C18G, Q160C mutations.PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1683
Plasmid#167622PurposeExpress TonB C18G, V206C.DepositorInserttonB C18G, V206C
UseTagsExpressionBacterialMutationtonB with C18G, V206C mutationsPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1362
Plasmid#167615PurposeExpress TonB C18G.DepositorInserttonB C18G
UseTagsExpressionBacterialMutationtonB with C18G mutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1684
Plasmid#167630PurposeExpress TonB C18G, R214C.DepositorInserttonB C18G, R214C
UseTagsExpressionBacterialMutationtonB with C18G, R214C mutationsPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP476
Plasmid#167594PurposeExpress TonB ∆(199-216)DepositorInserttonB ∆(199-216)
UseTagsExpressionBacterialMutationtonB ∆(199-216)PromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP372
Plasmid#167593PurposeExpress TonB frameshift of amphipathic helixDepositorInserttonB fs
UseTagsExpressionBacterialMutationtonB with frameshift of amphipathic helixPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-rrnOU
Plasmid#112785PurposeUpstream homology arm, rrnO-23S (23S rRNA)DepositorArticleInsertrrnOU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-amyEU
Plasmid#112781PurposeUpstream homology arm, amyE encodes _-amylaseDepositorArticleInsertamyEU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-P43
Plasmid#112775PurposeStrong promoter activity during exponential and stationary phaseDepositorArticleInsertP43 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_Empty
Plasmid#221480PurposeEmpty vector control for pLX307 overexpresion experimentsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
1_T7-mScarlet3-Hras
Plasmid#225928PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signalDepositorInsertmScarlet3-HRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203655PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen
Plasmid#218645PurposeGateway shuttling vector for APEX2-tagged P4C, a P4C biosensorDepositorInsertSidC(P4C)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_mCherry
Plasmid#221470PurposeGateway cloning of mCherry overexpressionDepositorInsertmCherry
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
2_T7-mScarlet3-KRas
Plasmid#225929PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signalDepositorInsertmScarlet3-KRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
5_T7-GAP43-mScarlet3
Plasmid#225932PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signalDepositorInsertGAP43-mScarlet3
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only