We narrowed to 1,254 results for: grna cloning vector

1. pCAGmCherry-gRNA

   Plasmid

   #87110

   Purpose

   gRNA cloning vector with CAGmCherry

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCY_gRNA_hPAX7-Pro_C6

   Plasmid

   #160457

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCY_gRNA_hPAX7-Pro_C5

   Plasmid

   #160456

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCY_gRNA_hPAX7-Pro_C4

   Plasmid

   #160455

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCY_gRNA_hPAX7-Pro_C3

   Plasmid

   #160454

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR-U6gRNA-PGKpuro2APDGFB

   Plasmid

   #136409

   Purpose

   Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BbsI restriction site in the PDGFB sequence (Glu1…

   Promoter

   U6, PDK

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR-U6gRNA-PGKpuro2ABFP

   Plasmid

   #136405

   Purpose

   Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BbsI restriction site at position 437 was removed…

   Promoter

   U6, PDK

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSK-mU6-Cj-sgRNA-SV40-puro

   Plasmid

   #192128

   Purpose

   Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Cj-SgRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-U6-sgRNA-PGK-puromycin-AflII

    Plasmid

    #106404

    Purpose

    Empty guide RNA cloning vector with an AflII site added from Addgene 41824

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gRNA_SRR124.5_tagBFP

    Plasmid

    #163753

    Purpose

    gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SRR124.5
    

    Use

    CRISPR

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. gRNA_SRR134.3_miRFP670

    Plasmid

    #163754

    Purpose

    gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SRR134.3
    

    Use

    CRISPR

    Tags

    miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSB700 lenti gRNA EBFP2_TtoC and hairpin extension

    Plasmid

    #167921

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB700 lenti gRNA Blasto_TtoC and hairpin extension

    Plasmid

    #167920

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB700 lenti gRNA EYFP_TtoC and hairpin extension

    Plasmid

    #167922

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB700 lenti gRNA Hygro_TtoC and hairpin extension

    Plasmid

    #167923

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB700 lenti gRNA iRFP713_TtoC and hairpin extension

    Plasmid

    #167924

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB700 lenti gRNA Puro_TtoC and hairpin extension

    Plasmid

    #167927

    Purpose

    Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge