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pTYF-Tretight-DsRed2

Plasmid

#19983

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

Tretight-DsRed2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flightless-1-LRR-HA

Plasmid

#21151

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

pcDNA3-Fli-Lrr-HA

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
Chicken ArcLight Q175

Plasmid

#53566

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-Q175

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND2-Right-G1397-C

Plasmid

#180771

Purpose

Expresses mitoZFD_ND2-Right-G1397-C(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND2 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 light chain

Plasmid

#198252

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/TRETightdU3

Plasmid

#18660

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pRIV-Gal4DBD:2xnMagHigh1

Plasmid

#182031

Purpose

Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1-p103'UTR

Use

Donor plasmid

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJL1-TTlight-4xDQNAT

Plasmid

#128394

Purpose

In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Use

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

T7

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397C-8e

Plasmid

#187170

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397C-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-8e-E1347A

Plasmid

#187166

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-8e-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-E1347A

Plasmid

#187164

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hpa5(aIIB_thigh)

Plasmid

#59971

Purpose

integrin a5 chimeric headpiece expression (a5 beta-propeller domain and aIIB thigh domain)

Depositor

Timothy Springer

Insert

human integrin a5 beta-propeller domain fused with human integrin aIIB thigh domain (ITGA5, ITGA2B Human)

Use

Tags

Acid coiled-coil and StrepII tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
Chicken ArcLight S174

Plasmid

#53567

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-S174

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Light chain

Plasmid

#216308

Purpose

Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V597C

Plasmid

#205080

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Insert

T7RNAP Cyslight V597C (T7p07 Bacteriophage T7)

Use

Tags

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V597C

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V166C

Plasmid

#205079

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Insert

T7RNAP Cyslight V166C (T7p07 Bacteriophage T7)

Use

Tags

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V166C

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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tl1-FusXTBE-RightTALE

Plasmid

#196507

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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