We narrowed to 382 results for: lentiviral construct

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CROPseq-multi-Zeo (pRW1445)

Plasmid

#216218

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistance

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD_IM

Plasmid

#84479

Purpose

Lentiviral construct to express dCas9 fused with an inactive catalytic domain of Tet1 (H1672Y, D1674A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH∆1

Plasmid

#41791

Purpose

Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

HIV-1 Gag/Pol and RRE sequences

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-LAP2β

Plasmid

#228029

Purpose

lentiviral construct for mRFP-LAP2β expression

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

LAP2b (Tmpo Rat)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

Plasmid

#225752

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

Plasmid

#225753

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAT031

Plasmid

#242611

Purpose

Empty MCP-fusion lentiviral construct for generating MCP-tethering RBP libraries in human cells

Depositor

Lacra Bintu

Article

Thurm et al bioRxiv [Preprint]. 2024 Jul 19:2024.07.

Type

Empty backbone

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_rtTA-P2A-BFP_HygroR

Plasmid

#186738

Purpose

rtTA-P2A-BFP expression construct cloned into lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

rtTA

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

Plasmid

#99144

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No3

Plasmid

#194898

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.3

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No9

Plasmid

#194903

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No4

Plasmid

#194899

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.4

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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