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Plasmid#28174DepositorInsertPP7 coat protein optimized
UseTags6xhistidine and triple FLAG peptideExpressionBacterialMutationC68A,C73APromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET29_LuxSit_i
Plasmid#194299PurposeBacterial expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET28c-Ku
Plasmid#161936PurposeExpresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purificationDepositorInsertku (mku Mycobacterium tuberculosis)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAR18_StrepI_mFASm_H8_pET22b
Plasmid#122847PurposeExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertmouse FASN/ mouse fatty acid synthase (Fasn Mouse)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a_PTK2b_Kinase
Plasmid#224242PurposeExpression of human PTK2b Kinase domain in E. coliDepositorInsertptk2b_kinase (PTK2B Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_MBP_RBFOX2
Plasmid#224292PurposeMBP-RBFOX2 protein purification from bacteriaDepositorInsertRNA binding fox-1 homolog 2 (RBFOX2 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H3
Plasmid#157807PurposeExpression of cysteine-free human histone H3 (c111A)DepositorInsertH3
UseTagsExpressionBacterialMutationC111APromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-petRNA
Plasmid#181802PurposepetRNA cloning plasmidDepositorInsertpetRNA-Kan
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-37
Plasmid#214346PurposeProtein overexpression in bacteriaDepositorInsertIL37 (IL37 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b_mNeonGreen
Plasmid#209425PurposeExpress mNeonGreen in E. coliDepositorInsertmNeonGreen
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM33_USP7_MATH
Plasmid#178490PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertUSP7_MATH (USP7 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EIF4E_eiF4E
Plasmid#178474PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertEIF4E_eiF4E (EIF4E Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b_Q69L
Plasmid#186272PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis-tagged at N terminalExpressionBacterialMutationChanged Q69 into LPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
FsC_pET29b
Plasmid#215405PurposeExpression construct for FsC gene, codon optimized for expression in E. coliDepositorInsertFsC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only