We narrowed to 223 results for: stem loop

1. ATF4 9: (SL+)5’ATF4.GFP

   Plasmid

   #21856

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   ATF4 (Atf4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Stem loop introduced at the 5' end of the mR…

   Available Since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET259-pUC57 24xMS2V6

   Plasmid

   #104391

   Purpose

   MS2V6 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET246-pUC57 12xMS2V6

   Plasmid

   #104390

   Purpose

   MS2V6 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 SREBF2

   Plasmid

   #67129

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SREBF2 (SREBF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pSB4C5_pBen-BenR

   Plasmid

   #128127

   Purpose

   Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   BenR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBen

   Available Since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET264-pUC 24xMS2V6 Loxp KANr Loxp

   Plasmid

   #104393

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET251-pUC 12xMS2V6 Loxp KANr Loxp

   Plasmid

   #104392

   Purpose

   Yeast endogenous mRNA tagging

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tagged-IRES

   Plasmid

   #35570

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase and MS2 Stem Loop

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

   Plasmid

   #86828

   Purpose

   Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-MS2V5-PABPC1 M161A/D165K

    Plasmid

    #65809

    Purpose

    expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC3 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    M161A/D165K

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-MS2V5-PABPC1 MLLEmut M161A/D165K

    Plasmid

    #65810

    Purpose

    expression of PABPC1-MS2 fusion, double mutant.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PABPC1 (PABPC4 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    MLLEmut M161A/D165K

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Expression

    Bacterial

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #104984

    Purpose

    Expresses ST-Renilla reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Tags

    24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNpF-∆7cg

    Plasmid

    #245371

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆7cg (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    7-bp deletion, H96Vfs*29, plus 2 synonymous mutat…

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZNpF-∆11cag

    Plasmid

    #245370

    Purpose

    Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

    Depositor

    Bruce Hamilton

    Article

    Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

    Insert

    Zfp423-∆11cag (Zfp423 Mouse)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    NanoLuciferase

    Expression

    Mammalian

    Mutation

    11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…

    Promoter

    EF1a

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. NLS-tdPCP-GFP-VP16

    Plasmid

    #183935

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP-VP16 (UL48 Synthetic)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CibN

    Plasmid

    #209671

    Purpose

    Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells

    Depositor

    Sanjeevi Sivasankar

    Article

    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Split-TurboID-N and pmEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119945

    Purpose

    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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