We narrowed to 13,583 results for: TIM

1. pIBA2-IntHA453-SpyTag

   Plasmid

   #198039

   Purpose

   Expression plasmid for producing SpyTagged intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Use

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   double HA tag inserted at position 453; lacks nat…

   Promoter

   tet

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBES1-LacOx50-blast

   Plasmid

   #210221

   Purpose

   Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   50xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pATT-BsaI-Pmag-NmagHigh1

   Plasmid

   #202713

   Purpose

   Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

   Depositor

   Mary Dunlop

   Article

   Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

   Insert

   linker-pMag-RBS-nMagHigh1-linker
   

   Use

   Staging plasmid to make transposon insertion libr…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pATT-Dest-Cre

   Plasmid

   #202714

   Purpose

   Cre recombinase staging plasmid to make transposon insertion libraries.

   Depositor

   Mary Dunlop

   Article

   Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

   Insert

   Cre
   

   Use

   Staging plasmid to make transposon insertion libr…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTKEI-Dest-loxP-sfGFP

   Plasmid

   #202715

   Purpose

   Empty expression vector containing Cre recombinase reporter.

   Depositor

   Mary Dunlop

   Article

   Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

   Insert

   Pw7-loxP-term-loxP-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. csr-1_son-1_sGFP

   Plasmid

   #191561

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and sGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. csr-1_son-1_mRuby3

   Plasmid

   #191744

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mRuby3

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. csr-1_son-1_mNeptune2.5

   Plasmid

   #191748

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mNeptune2.5

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFPO-His-mTurquoise2_T203Y

   Plasmid

   #191456

   Purpose

   Bacterial isolation of mTurquoise2 with a T203Y mutation

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   mTurquoise2_T203Y
   

   Use

   Tags

   His

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_STf

    Plasmid

    #182000

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_STf_SKL

    Plasmid

    #182007

    Purpose

    CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    STf_SKL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIS.MTRIA-PAF

    Plasmid

    #184616

    Purpose

    For expression of a fluorescent sensor for platelet-activating factor in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-PAF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIS.MTRIA-UTS

    Plasmid

    #184620

    Purpose

    For expression of a fluorescent sensor for urotensin II in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-UTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIS.MTRIA-LTB

    Plasmid

    #184605

    Purpose

    For expression of a fluorescent sensor for leukotriene B4 in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-LTB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCIS.MTRIA-NKB

    Plasmid

    #184609

    Purpose

    For expression of a fluorescent sensor for neurokinin B in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NKB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIS.MTRIA-C3a

    Plasmid

    #184601

    Purpose

    For expression of a fluorescent sensor for C3a complement in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-C3a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCIS.MTRIA-NTS

    Plasmid

    #184614

    Purpose

    For expression of a fluorescent sensor for neurotensin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIS.MTRIA-NPFF

    Plasmid

    #184612

    Purpose

    For expression of a fluorescent sensor for neuropeptide FF in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NPFF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIS.MTRIA-NMB

    Plasmid

    #184610

    Purpose

    For expression of a fluorescent sensor for neuromedin B in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NMB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMSCV-IRES-GFP-polar-bear-Caspase-1/4b

    Plasmid

    #183370

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4b
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiguide-puro_hNKD2sg#3

    Plasmid

    #174325

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLentiguide-puro_hNKD2sg#2

    Plasmid

    #174324

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLentiguide-puro_hNKD2sg#1

    Plasmid

    #174323

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid

    #174138

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid

    #174139

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBD21_035

    Plasmid

    #109152

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. C453-E04: attB4-CMV51p>-attB5

    Plasmid

    #162973

    Purpose

    Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    optimized human CMV promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. C413-E36: attB4-CMV51p>-attB1r

    Plasmid

    #162927

    Purpose

    Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    optimized CMV promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFapO_94

    Plasmid

    #149597

    Purpose

    pXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFP

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    yeGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1-FapO_94

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXylO_94

    Plasmid

    #149598

    Purpose

    pXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFP

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    yeGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1-XylO_94

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge