We narrowed to 12,157 results for: pcdna

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pcDNA3-FLAG-Luc-N

Plasmid

#58790

Purpose

Express LucN alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 1-398

Tags

FLAG

Expression

Mammalian

Mutation

T576C,T591C to destroy MfeI and EcoRI sites

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Luc-C

Plasmid

#58791

Purpose

Express LucC alone in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

firefly Luciferase 394-550

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA4/TO-GFP-SPOP (WT)

Plasmid

#197008

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

-

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP-PKD2

Plasmid

#31821

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-Protein Kinase D2 (PRKD2 Human)

Tags

YFP

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-HA-mRFP-pcDNA3.1-

Plasmid

#52510

Purpose

expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

mRFP

Tags

FLAG-HA

Expression

Mammalian

Mutation

engineered variant of tetrameric red fluorescent …

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-KIAA0947

Plasmid

#49248

Purpose

Mammalian expression of flag-tagged human KIAA0947

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

KIAA0947 (ICE1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-HtrA2-EGFP

Plasmid

#14121

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2002 Jan 4. 277(1):439-44.

Insert

HtrA2 (HTRA2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1+_FH-AGO2-WT

Plasmid

#92006

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PDI-QUAD-V5-pcDNA3.1

Plasmid

#153550

Purpose

PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.

Depositor

Julie Atkin

Article

Parakh et al iScience. 2020 May 22;23(5):101097. doi:

Insert

Protein disulphide isomerase

Tags

V5 tag

Mutation

C53S, C56S, C397S, C400S

Promoter

pCMV

Available Since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 proHB-EGF CS

Plasmid

#11601

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Tags

His and myc

Expression

Mammalian

Mutation

Constitutively secreted HB-EGF: C-terminal amino …

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-L530P

Plasmid

#32710

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

L530P

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/V5-His-mPer1

Plasmid

#219421

Purpose

Expresses 3873 bp of mPer1 coding sequence in E. coli.

Depositor

Aziz Sancar

Article

Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mPer1 (Per1 Mouse)

Tags

His and V5

Expression

Bacterial and Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT

Plasmid

#112705

Purpose

Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LTK-V5/HIS

Plasmid

#200116

Purpose

Expression of human LTK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human LTK receptor tyrosine kinase (LTK Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PalmPalm-ClopHensor

Plasmid

#25940

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

PalmPalm-ClopHensor

Tags

Gap20 and Strep-II

Expression

Mammalian

Mutation

N-term (20AA) of Gap-43

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mArf1(Q71L)-HA

Plasmid

#79410

Purpose

Expresses C-terminally HA-tagged Arf1(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (Arf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_LamB1

Plasmid

#181999

Purpose

CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf-LamB1

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC4.3SA-FLAG

Plasmid

#30486

Depositor

Tso-Pang Yao

Insert

HDAC4 (HDAC4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S246/467/632A

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF73-2xCSTREP

Plasmid

#136232

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF73

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y397F

Plasmid

#50503

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y397F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HA-mSTING (V154M)

Plasmid

#214159

Purpose

Transient expression of gain-of-function STING mutant

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (V154M) (Sting1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

N153S

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-VSVG

Plasmid

#80606

Purpose

Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

VSV-G

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf3(WT)-mCherry

Plasmid

#79420

Purpose

Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf3 (ARF3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP1

Plasmid

#18978

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

His

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB6b

Plasmid

#19528

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB6b (DNAJB6 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 2x (S2x)

Plasmid

#37004

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 2x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Two copies of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-K179M

Plasmid

#73409

Purpose

Expresses kinase-inactive mutant of Akt with HA tag

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

changed K179M to make Kinase inactive mutant

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA Set9 mutant

Plasmid

#24085

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF50-2xCSTREP

Plasmid

#136211

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF50

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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