We narrowed to 12,475 results for: pcdna

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pcDNA3/RGS-UBC12

Plasmid

#20080

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 1999 Apr 23;274(17):12036-4

Insert

UBC12 (UBE2M Human)

Tags

6xHis

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hyg-mEGFP

Plasmid

#191847

Purpose

Expresses the monomeric form of EGFP

Depositor

Bertrand Collet

Article

Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

Insert

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Ufd1-Strep-HA

Plasmid

#113474

Purpose

Expression of human Ufd1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Ufd1 (UFD1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX53-FLAG

Plasmid

#222241

Purpose

Express FLAG tagged DDX53 in mammalian cells

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

T7

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX53-GFP

Plasmid

#222239

Purpose

Express GFP tagged DDX53 in mammalian cells

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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IFNAR2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176938

Purpose

Mammalian expression vector encoding IFNAR2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

IFNAR2 (IFNAR2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin WT

Plasmid

#50853

Purpose

expresses human cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag JIP3b

Plasmid

#53458

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2-WT(732)-8h

Plasmid

#154101

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (8his tag)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-10nm-EBFP2

Plasmid

#231184

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

10nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer

Plasmid

#174394

Purpose

Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain with leucine zipper & C-C linker

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-GreenPy1Low

Plasmid

#239071

Purpose

Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Low

Expression

Mammalian

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut2

Plasmid

#18086

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut2 (SLC2A2 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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DOP1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176979

Purpose

Mammalian expression vector encoding DOP1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DOP1B (DOPEY2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-V5-B/BRISBANE/60/2008

Plasmid

#187923

Purpose

Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Tags

V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag

Plasmid

#112487

Purpose

Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT2

Plasmid

#53555

Purpose

mammalian expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.1 (pcDNA3.1+)

Plasmid

#75190

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4-NLS

Plasmid

#138217

Purpose

Nuclear-targeted FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

AKAR4-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi2-91-NLuc

Plasmid

#207149

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI2 (GNAI2 Human)

Expression

Mammalian

Mutation

NLuc inserted at position L(91)Q

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc card9

Plasmid

#16253

Depositor

William Kaelin

Article

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 (CARD9 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA3-TSC1

Plasmid

#19911

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL

Plasmid

#24233

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-EGFP-GAPDH

Plasmid

#225475

Purpose

Express mEGFP-fusion protein of GAPDH

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ha-Brca1 S1423A

Plasmid

#32533

Depositor

Michael Kastan

Article

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

BRCA1 (BRCA1 Human)

Tags

HA

Expression

Mammalian

Mutation

S1423A

Promoter

CMV/rabbit B-globin

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HA-SrtAstrep (pKS77)

Plasmid

#50030

Purpose

Expression of cytosolic S. pyogenes SrtA in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Tags

HA

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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