We narrowed to 18,610 results for: RAN-1

1. pTALEN_v2 (NN)

   Plasmid

   #32191

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   TALE Nuclease, NN for 0.5 repeat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMOD4 RT-G

   Plasmid

   #19184

   Depositor

   Al Fisher

   Article

   Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

   Insert

   rpsL tetA
   

   Use

   Recombineering

   Tags

   50 nucleotides of FLAG-GFP

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pM12-ISH

   Plasmid

   #15954

   Depositor

   Peter Mombaerts

   Article

   Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

   Insert

   M12 (Olfr19 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pgk-ATG-frt

   Plasmid

   #20734

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   pgk-ATG-frt-SAdpA-tetO
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJMP2846

   Plasmid

   #160676

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKmyc-Exp5

   Plasmid

   #12552

   Depositor

   Ian Macara

   Article

   Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

   Insert

   Exportin-5 (XPO5 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Oct. 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJMP2844

   Plasmid

   #160675

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRM2

   Plasmid

   #8486

   Depositor

   Nancy Craig

   Article

   McKown et al J Bacteriol 1988 Jan;170(1):352-8.

   Insert

   attTn7 (tnsA Bacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK2-let-7 4x

   Plasmid

   #20930

   Depositor

   Yukihide Tomari

   Article

   Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

   Insert

   4x let-7 target sites
   

   Use

   Luciferase

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJMP2849

    Plasmid

    #160677

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCHECK2-let-7 2x

    Plasmid

    #20929

    Depositor

    Yukihide Tomari

    Article

    Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

    Insert

    2x let-7 target sites
    

    Use

    Luciferase

    Tags

    Expression

    Insect and Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pJMP2782

    Plasmid

    #160668

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC1809_pmax-RBM38-FRB

    Plasmid

    #118642

    Purpose

    Transient transfection; Expresses RBM38-FRB; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBM38-FRB
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC1810_pmax-FRB-RBM38

    Plasmid

    #118643

    Purpose

    Transient transfection; Expresses FRB-RBM38; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    FRB-RBM38
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGST1-Osh4 (2-434)

    Plasmid

    #17632

    Depositor

    James Hurley

    Article

    Im et al Nature. 2005 Sep 1. 437(7055):154-8.

    Insert

    Osh4 (KES1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pM5HHB5

    Plasmid

    #17674

    Depositor

    David Shalloway

    Article

    Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

    Insert

    c-src (SRC Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

17. Vg4-Luc

    Plasmid

    #16501

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Mad-binding element from Vg
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pS1-ISH

    Plasmid

    #15516

    Depositor

    Peter Mombaerts

    Article

    Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

    Insert

    S1 (Olfr749 Mouse)
    

    Use

    For riboprobe, in situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pPS1889

    Plasmid

    #1361

    Depositor

    Pamela Silver

    Article

    Damelin et al Mol Cell 2000 Jan;5(1):133-40.

    Insert

    NLS-ECFP-EYFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pSC101-AmpR EV

    Plasmid

    #208184

    Purpose

    empty plasmid vector, non-fluorescent

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Type

    Empty backbone

    Use

    Reporter

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMOD4 galK-G

    Plasmid

    #19182

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    galK (galK E.coli)
    

    Use

    Recombineering

    Tags

    50 nucleotides of FLAG-GFP

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQE60-Exp5

    Plasmid

    #12553

    Depositor

    Ian Macara

    Article

    Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

    Insert

    EXPORTIN-5 (XPO5 Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    NONE

    Promoter

    Available Since

    Oct. 2, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pCD061

    Plasmid

    #113315

    Purpose

    W108 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA.b1_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCD315

    Plasmid

    #113321

    Purpose

    pBAD-W108 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    scRNA.b1_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKL08

    Plasmid

    #104432

    Purpose

    pHuji in yeast expression vector

    Depositor

    Joel Kralj

    Article

    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

    Insert

    pHuji
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pISH-mCherry

    Plasmid

    #105481

    Purpose

    to generate a riboprobe for in situ hybridazation

    Depositor

    Peter Mombaerts

    Article

    Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

    Insert

    mCherry
    

    Use

    To ganerate riboprobe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCD005

    Plasmid

    #113314

    Purpose

    W108 scRNA

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCB116

    Plasmid

    #53365

    Purpose

    To express Cup9p‐FLAG from the PGAL1 promoter

    Depositor

    Alexander Varshavsky

    Article

    Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

    Insert

    Cup9 (CUP9 Budding Yeast)
    

    Use

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pCB210

    Plasmid

    #31722

    Purpose

    To express Cup9p‐FLAG from the PCUP9 promoter

    Depositor

    Alexander Varshavsky

    Article

    Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

    Insert

    CUP9 (CUP9 Budding Yeast)
    

    Use

    Tags

    Cup9 promoter and FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pCMK103

    Plasmid

    #226803

    Purpose

    Integrates KanR into NS1, 500 bp homology, one homologous HIP1 site in left homology arm

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMK104

    Plasmid

    #226804

    Purpose

    Integrates KanR into NS2, 500 bp homology, one homologous HIP1 site in right homology arm

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMK105

    Plasmid

    #226805

    Purpose

    Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm

    Depositor

    David Nielsen

    Article

    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHC_O2

    Plasmid

    #205224

    Purpose

    part of Bactoneuron DF2, expresses mvenus

    Depositor

    Sangram Bagh

    Article

    Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHTC_O3

    Plasmid

    #205225

    Purpose

    part of Bactoneuron DF4, expresses mTagBFP2

    Depositor

    Sangram Bagh

    Article

    Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAC_O3

    Plasmid

    #205226

    Purpose

    part of Bactoneuron DF5, expresses mTagBFP2 and LuxR

    Depositor

    Sangram Bagh

    Article

    Srivastava et al ACS Synth Biol. 2023 Jan 20;12(1):51-60.

    Insert

    mTagBFP2, LuxR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBH502

    Plasmid

    #215781

    Purpose

    NBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting Nanoluc

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Inserts

    LacI(YQR)
    sgRNA-ribozyme cassette
    Nanoluc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bt1311, P(O1), and cfxA

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. L0-T3 mTurq-tUBQ10

    Plasmid

    #195886

    Purpose

    Level 0 mTurq-tUBQ10 for integrase target construction

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    mTurq-UBQ10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-S*HiGlpG

    Plasmid

    #158534

    Purpose

    Bacterial expression of rhomboid protease mutant S*HiGlpG

    Depositor

    Sinisa Urban

    Article

    Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

    Insert

    S*HiGlpG
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Sector 1 and 2 mutations

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-S1*HiGlpG

    Plasmid

    #158535

    Purpose

    Bacterial expression of rhomboid protease mutant S1*HiGlpG

    Depositor

    Sinisa Urban

    Article

    Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

    Insert

    S1*HiGlpG
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Sector 1 mutations

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. (-6kb/-207)mtH4-GFP

    Plasmid

    #30464

    Depositor

    Gerhart Ryffel

    Article

    Ryffel et al Mech Dev. 2000 Jan . 90(1):65-75.

    Insert

    HNF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of HNF4 binding site at 5471-5494 (Holew…

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. (-6kb/-58)mtH4-GFP

    Plasmid

    #30463

    Depositor

    Gerhart Ryffel

    Article

    Ryffel et al Mech Dev. 2000 Jan . 90(1):65-75.

    Insert

    HNF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of HNF4 binding site at 5471-5494 (Holew…

    Promoter

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

42. PGK-GFP-LAMP1TM

    Plasmid

    #234874

    Purpose

    Lentiviral plasmid expressing GFP fused to the transmembrane domain and cytosolic tail of LAMP-1. Designed as a control vector for PGK-HA-PGRN-LAMP1TM.

    Depositor

    Andrew Arrant

    Article

    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    LAMP-1 transmembrane domain and cytosolic tail

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBRIT Pax7 FLAG

    Plasmid

    #17521

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

44. pBRIT Pax7 TAP

    Plasmid

    #17528

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

45. pTH730-staRLuc

    Plasmid

    #38226

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 (=GPD)

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Zhi3-mCherry

    Plasmid

    #106295

    Purpose

    CMV driven mCherry, zeocin resistance

    Depositor

    Sanford Simon

    Article

    Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pDmDERusk

    Plasmid

    #213702

    Purpose

    Vector for red-light-induced bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pDmREDusk

    Plasmid

    #213701

    Purpose

    Vector for red-light-repressed bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pDrDERusk

    Plasmid

    #213703

    Purpose

    Vector for red-light-induced bacterial gene expression

    Depositor

    Andreas Moeglich

    Article

    Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCD185

    Plasmid

    #113317

    Purpose

    dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    R93A

    Promoter

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge