We narrowed to 4,099 results for: LYC

1. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

   Plasmid

   #222917

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrc99S-ssDsbA-MBP-4xDQNAT

   Plasmid

   #128398

   Purpose

   Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Y342H- please see depositor comments

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKG-GW1

   Plasmid

   #203163

   Purpose

   Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBP-2_6.sensor

   Plasmid

   #162800

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   2_6_RNA-device
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Man2a1-myc

   Plasmid

   #163648

   Purpose

   Expresses myc tagged Man2a1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mannosidase alpha class 2A member 1 (MAN2A1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Silent mutation was made to introduce a XbaI rest…

   Promoter

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti hSyn AldoA

   Plasmid

   #220916

   Purpose

   Neuronal specific expression of AldoA

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   AldoA (ALDOA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. (318) pcDNA3.1-myc-OGA(1-400)D174N

   Plasmid

   #168096

   Purpose

   Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   myc-OGA(1-400, D174N)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D174N catalytically inactive OGA

   Promoter

   T7/CMV

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2 Smad1 EVE GM

   Plasmid

   #27997

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   Human Smad1 EVE Gsk3 mutant (SMAD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S463E S465E S210A T202A S191A S198A

   Promoter

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

   Plasmid

   #128404

   Purpose

   Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A (toxA P. aeruginosa)
   

   Use

   Tags

   6xHis tag and DsbA signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFIV-H1-Puro-hPolß

    Plasmid

    #18663

    Depositor

    Robert Sobol

    Article

    Trivedi et al Molecular Pharmacology 2008;74(2):505-16

    Insert

    shRNA specific to human DNA polymerase beta (POLB Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Oct. 24, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pHDR_NANOS3-T2A-mVenus-PuroTK

    Plasmid

    #222903

    Purpose

    Homology directed repair template for knocking in mVenus reporter to NANOS3.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mVenus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid

    #128402

    Purpose

    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

    Plasmid

    #221080

    Purpose

    SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SBP-ST*-GFP

    Plasmid

    #163668

    Purpose

    Expresses SBP tagged partial length ST in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    

    Use

    Tags

    GFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    This chimera contains the first 113 amino acids o…

    Promoter

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. LENTICRISPR-PGAM1_sgRNA1

    Plasmid

    #201614

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3XFlag-Mettl4

    Plasmid

    #86665

    Purpose

    Expresses murine Mettl4 containing N-terminal 3XFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Mettl4 (Mettl4 Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-SARS2-Spike-D614G

    Plasmid

    #166850

    Purpose

    For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

    Depositor

    Neville Sanjana

    Article

    Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2 virus)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. p3xFlag-Alkbh4

    Plasmid

    #105240

    Purpose

    Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Alkbh4 (Alkbh4 Mouse)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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