We narrowed to 7,454 results for: ef1a

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pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

Plasmid

#117137

Purpose

Expression vector encoding BFP-BSD-Nkx2-5

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

Plasmid

#113155

Purpose

An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat TH

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

Plasmid

#113157

Purpose

An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat MANF

Use

AAV and CRISPR

Expression

Mammalian

Promoter

mU6 and hU6

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha/TRE3G

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

Plasmid

#188766

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro

Plasmid

#248161

Purpose

Expresses KRAB fused to dCas9 and BFP upon cumate induction

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

KRAB-dCas9-HA-tagBFP
HA-CymR-T2A-puro

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

Plasmid

#188778

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

Plasmid

#186980

Purpose

mammalian expression of Cluc-Gluc reporter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

Cluc, Gluc

Use

Luciferase

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

Plasmid

#175571

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pXD023 - pLKO-EF1a-Puro-P2A-FLuc-WPRE

Plasmid

#192203

Purpose

Lenti-PL(Puro-Luciferase)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-PL(Puro-Luciferase)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

Plasmid

#103865

Purpose

Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

Contains the H133A and H1058A mutations which ina…

Promoter

EF1a

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTY-EF1a-Hoxa9-p2a-MeisI-p2a-GFP

Plasmid

#61738

Purpose

Express Hoxa9 and MeisI to induce leukemic transformation. GFP label transduced cells.

Depositor

Yi Zhang

Article

He et al Blood. 2011 Apr 7;117(14):3869-80. doi:

Insert

Hoxa9-MeisI-GFP

Use

Lentiviral

Promoter

EF1a

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-MeCP2-H2B-GFP

Plasmid

#242170

Purpose

Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-GFP reporter

Depositor

Amy Brock

Insert

dCas9-KRAB-MeCP2; T2A-H2B-GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

Plasmid

#192204

Purpose

Lenti-GL(GFP-Luciferase)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-GL(GFP-Luciferase)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

Plasmid

#193469

Purpose

Cp36 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

Plasmid

#184635

Purpose

Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-FastFUCCI-IRES-3xnls-mTagBFP2 (JDW 1511)

Plasmid

#242599

Purpose

A PiggyBac expression vector containing the human EF1a promoter driving FastFUCCI to label cell cycle state followed by an IRES-3xnls-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110) (EEF1A1 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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