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Showing: 1581 - 1600 of 1671 results

  1. BFP-RanQ69L

    Plasmid
    #163438
    Purpose
    Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation
    Depositor
    Sanford Simon
    Insert
    RanQ69L (RAN Human)
    Use
    Tags
    tag-BFP
    Expression
    Mammalian
    Mutation
    Q69L
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163438-plasmid-map-sequence-id-321655

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  2. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid
    #207636
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207636-plasmid-map-sequence-id-409876

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  3. pYL236

    Plasmid
    #173184
    Purpose
    CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene
    Depositor
    Thomas Cech
    Article
    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:
    Insert
    EZH2 (EZH2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173184-plasmid-map-sequence-id-341220

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  4. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid
    #184636
    Purpose
    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion
    Depositor
    Nicolas Tritsch
    Article
    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:
    Insert
    Gat1 (Slc6a1 Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Gat1 gene with Arginine 69 changed to Lysine, to …
    Promoter
    EF1a
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184636-plasmid-map-sequence-id-369063

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  7. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  8. pFUGW-FIRE-pHLy

    Plasmid
    #170774
    Purpose
    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    hUbC
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    170774-plasmid-map-sequence-id-339008

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  16. pAAV-U6-racRNA-A

    Plasmid
    #200824
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200824-plasmid-map-sequence-id-396714

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  17. pAAV-U6-racRNA-B

    Plasmid
    #200825
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200825-plasmid-map-sequence-id-396771

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  18. pAAV-U6-racRNA-C

    Plasmid
    #200826
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200826-plasmid-map-sequence-id-396715

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  19. pAAV-U6-racRNA-D

    Plasmid
    #200827
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200827-plasmid-map-sequence-id-396773

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Showing: 1581 - 1600 of 1671 results