We narrowed to 60,551 results for: promoter

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GFP-UBR5

Plasmid

#52050

Purpose

CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

Depositor

Darren Saunders

Article

Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

Insert

UBR5 (UBR5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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HA-Sos1

Plasmid

#32920

Depositor

Dafna Bar-Sagi

Article

Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

Insert

SOS1 (SOS1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2

Plasmid

#18047

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SomaGCaMP6f2

Plasmid

#158757

Purpose

Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Has Service

AAV8 and AAV9

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3

Plasmid

#189754

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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LifeAct-mNeonGreen

Plasmid

#98877

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LifeAct

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, William Hahn, David Root

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFC332

Plasmid

#87845

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

Depositor

Uffe Mortensen

Article

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
hph (hygromycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-CRY2-CreN

Plasmid

#26888

Purpose

Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2-CreN (cre, CRY2 Mustard Weed)

Expression

Mammalian

Mutation

aa 19-104 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-ABCG2-IRES-Neo

Plasmid

#25983

Depositor

Ren-he Xu

Article

Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

Insert

ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)

Use

Lentiviral

Tags

EF1-apha promoter and IRES-Neo

Expression

Mammalian

Mutation

None

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer1

Plasmid

#14763

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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4xmts-mScarlet-I

Plasmid

#98818

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

mts

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

hrGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Bx

Plasmid

#51552

Purpose

Expresses Bxb1 integrase in mammalian cells.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

Bxb1 integrase

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2-N

Plasmid

#41645

Purpose

Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

mNeptune2-Dronpa145N

Expression

Mammalian

Mutation

K145N on Dronpa (allows control of photochromic F…

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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