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Lenti_CRISPRi_sgAR10

Plasmid

#167003

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 10 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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EC67842

Plasmid

#209516

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mouse b8 full length

Plasmid

#217817

Purpose

Full length mouse integrin b8 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB8 (Itgb8 Mouse)

Tags

P2A-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGEN

Plasmid

#11160

Purpose

Mammalian expression vector for cloning and expressing your gene under the CAG promoter

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

modified multiple cloning sites

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5

Plasmid

#13095

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5 (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pEND-184_Cacnes_mCherry

Plasmid

#225610

Purpose

Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mCherry

Use

Synthetic Biology

Tags

V5-6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

Plasmid

#210017

Purpose

episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Expression

Mammalian

Mutation

Sox2A61V is a point mutant of Sox2 that enhances …

Promoter

CAG

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_WDHD1:1-1129

Plasmid

#211175

Purpose

Mammalian expression of full-length human WDHD1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDHD1:M1-E1129 (WDHD1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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