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pUC-UAS1B4-Leum

Plasmid

#44312

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B4-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B4-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p1.1

Plasmid

#162737

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Talin-L432G

Plasmid

#26725

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 432 to Glycine to block ability o…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(504)

Plasmid

#44372

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(504) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(504)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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ykgMO-IGS-LuxCDABE

Plasmid

#207232

Purpose

Calprotectin sensing promoter with luciferase reporter

Depositor

Arthur Prindle

Article

Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

ykgMO-IGS

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B28-Leum

Plasmid

#44327

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B28-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B28-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B16-Leum

Plasmid

#44324

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-Leum

Available since

May 23, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(203)

Plasmid

#44377

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(203) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(203)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-Cre

Plasmid

#122562

Purpose

Integration of Cre sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-Cre-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-Leum

Plasmid

#44316

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF

Plasmid

#44374

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B20-Leum

Plasmid

#44325

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B20-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B20-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pJK371

Plasmid

#73596

Purpose

Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

Depositor

T. J. Kappock

Inserts

delta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)

Use

Tags

FLAG tag and c-myc tag

Expression

Yeast

Mutation

Point mutants in FAD2-3 ORF relative to deposited…

Promoter

GAL1 and GAL10

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(1004)

Plasmid

#44365

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(1004) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pFGL1344

Plasmid

#116902

Purpose

Alp6:Bar:mCherry (SPB/MTOC marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Alp6 (partial ORF)
Alp6 downstream region

Use

Fungal expression (in magnaporthe oryzae)

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge