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Addgene

We narrowed to 4,048 results for: GAST

Showing: 4001 - 4048 of 4048 results
  1. pJAT62

    Plasmid
    #204301
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pJAT56

    Plasmid
    #204300
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pJAT17

    Plasmid
    #204298
    Purpose
    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.
    Depositor
    Insert
    3XP3-EYFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pLib6.6

    Plasmid
    #176652
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pLib6.4B

    Plasmid
    #176665
    Purpose
    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pLib6.6B

    Plasmid
    #176666
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pGEM-WingGFP-tan

    Plasmid
    #83473
    Purpose
    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Insert
    WingGFP-tan
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Hsp70 and enhancer from D. melanogaster yellow ge…
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pKM60

    Plasmid
    #154159
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 wt NF-κB sites were replaced with Rel1A-specifi…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pAA3

    Plasmid
    #154163
    Purpose
    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    20 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pAA2

    Plasmid
    #154162
    Purpose
    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    16 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pAA1

    Plasmid
    #154161
    Purpose
    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    12 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pKM62

    Plasmid
    #154160
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 wt NF-κB sites were replaced with Rel2-specific…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid
    #218863
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    Insert
    RSET-jGCaMP8m
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    A25G F286Y
    Promoter
    hsp70
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid
    #218864
    Purpose
    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    Insert
    RSET-jGCaMP8s
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    S26M F286Y Q315H
    Promoter
    hsp70
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid
    #218867
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    Insert
    RSET-jGCaMP8f
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    Q315L
    Promoter
    hsp70
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid
    #218865
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    Insert
    RSET-jGCaMP8s
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    S26M F286Y Q315H
    Promoter
    hsp70
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GIPR (GIPR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. GRPR-DuET

    Plasmid
    #213306
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GRPR (GRPR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pcDNA3.1-PDGFRA-V561D-V5/HIS

    Plasmid
    #234762
    Purpose
    Expression of the V561D mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor and GIST-Plus Syndrome, , constantly activated
    Depositor
    Insert
    PDGFRA-V561D receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    V561D substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. pcDNA3.1-PDGFRA-R487L-V5/HIS

    Plasmid
    #234761
    Purpose
    Expression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal Tumor
    Depositor
    Insert
    PDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    R487L substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. pcDNA3.1-PDGFRA-D842V-V5/HIS

    Plasmid
    #234763
    Purpose
    Expression of the D842V mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor, constantly activated
    Depositor
    Insert
    PDGFRA-D842V receptor tyrosine kinase, full length (PDGFRA Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D842V substitution
    Promoter
    CMV
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. pEMS1496

    Plasmid
    #29244
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Insert
    Ple25 (CCKBR Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
  23. pEMS1495

    Plasmid
    #29243
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).
    Depositor
    Insert
    Ple24 (CCKBR Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    Nov. 7, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4001 - 4048 of 4048 results