We narrowed to 4,823 results for: donor

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pUC19-TgKI-MCS-mScarlet-MCS-polyA

Plasmid

#174023

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mScarlet

Use

Zebrafish knock-in tagging

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

Plasmid

#174029

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-mScarlet

Use

Zebrafish knock-in tagging

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTopo pLox hPGK-Puro pA pLox

Plasmid

#171048

Purpose

Empty donor template for CRISPR/Cas9 targeting of TERT RE's

Depositor

Agnel Sfeir

Publication

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDD399

Plasmid

#91821

Purpose

AID CT-tag linker donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139485

Purpose

PCR template for Halo-Tag gene knock-in donor vector

Depositor

Kazuhiro Aoki

Publication

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Insert

HaloTag-loxP-P2A-dTKneo-pA-loxP

Tags

HaloTag

Expression

Mammalian

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP

Plasmid

#139484

Purpose

PCR template for mEGFP gene knock-in donor vector

Depositor

Kazuhiro Aoki

Publication

Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mEGFP-loxP-P2A-dTKneo-pA-loxP

Tags

mEGFP

Expression

Mammalian

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AD

Plasmid

#108687

Purpose

Plasmid for assembly of donor DNA templates consisting of three expression units

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-NLS-actin-R62D

Plasmid

#118381

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS and R62D

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC1816_pCR8-gSMN2-DN4

Plasmid

#118649

Purpose

Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gSMN2-DN4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLHDR03_mCherry_tCD19_unique guide

Plasmid

#124062

Purpose

Construction of 1st donor for scarless genome editing with unique guide

Depositor

Matthew Porteus

Publication

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

mCherry
tCD19

Use

Mouse Targeting; Human targeting

Promoter

Human UbC modified

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-PKCa-mEGFP-N1

Plasmid

#112269

Purpose

IDOCKSalpha Donor Construct

Depositor

Ryohei Yasuda

Publication

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Protein Kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TCF4 - P381L in pENTR

Plasmid

#216570

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway Cloning

Mutation

P381L

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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TCF4 - D424N in pENTR

Plasmid

#216571

Purpose

gateway cloning donor vector containing TCF4 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

TCF4 (TCF4 Human)

Use

Gateway Cloning

Mutation

D424N

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - G752A in pENTR

Plasmid

#216623

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

G752A

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R673w in pENTR

Plasmid

#216622

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

R673W

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - T551M in pENTR

Plasmid

#216621

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

T551M

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R565C in pENTR

Plasmid

#216620

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

R565C

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - R376H in pENTR

Plasmid

#216619

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

R376H

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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CTNNB1 - W388S in pENTR

Plasmid

#216618

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway Cloning

Mutation

W388S

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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