12,610 results

1. pGL3B-1081

   Plasmid

   #18759

   Depositor

   Clark Distelhorst

   Article

   Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

   Insert

   thioredoxin-interacting protein promoter (Txnip Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

   Plasmid

   #212705

   Purpose

   targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

   Depositor

   Elzo de Wit

   Insert

   ZFP143-FKBPV-2HA-P2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #218634

   Purpose

   Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   delPYD (I125M-END)

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE puro PPAR gamma 2

   Plasmid

   #8859

   Depositor

   Bruce Spiegelman

   Article

   Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

   Insert

   PPAR gamma (Pparg Mouse)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGW1-Myc-Sarm1

   Plasmid

   #50707

   Purpose

   expression of Sarm1 in mammalian cells

   Depositor

   Yi-Ping Hsueh

   Article

   Chen et al J Cell Biol. 2011 May 16;193(4):769-84.

   Insert

   Sarm1 (Sarm1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-SUN1 mScarlet

   Plasmid

   #187683

   Purpose

   In vivo visualization of SUN1

   Depositor

   Mary Beckerle

   Insert

   SUN1 (Sun1 Mouse)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMK445 (BSD-P2A-BromoTag)

   Plasmid

   #214375

   Purpose

   BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-Hb9-RFP

   Plasmid

   #37081

   Depositor

   Fred Gage

   Article

   Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

   Insert

   Hb9 RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Hb9

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BK alpha subunit

   Plasmid

   #113566

   Purpose

   mouse BK channel alpha subunit expression vector

   Depositor

   Robert Brenner

   Article

   Gruslova et al J Gen Physiol. 2012 Jan;139(1):57-67. do

   Insert

   Kcnma1 (Kcnma1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-EGFP-Rheb-IP

    Plasmid

    #13831

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    Rheb (Rheb Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pBluescript-mouse cyclin D2

    Plasmid

    #86791

    Purpose

    bacterial expression of mouse cyclin D2

    Depositor

    Martine Roussel, Charles Sherr

    Article

    Matsushime et al Cell. 1991 May 17;65(4):701-13.

    Insert

    cyclin D2 (Ccnd2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Capping Protein

    Plasmid

    #89950

    Purpose

    F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.

    Depositor

    Guillaume Romet-Lemonne, Antoine Jégou

    Article

    Shekhar et al Nat Commun. 2015 Nov 13;6:8730. doi: 10.

    Inserts

    F-actin-capping protein subunit alpha-1 (Capza1 Mouse)
    F-actin-capping protein subunit beta (Capzb Mouse)
    

    Use

    Tags

    6*His

    Expression

    Bacterial

    Mutation

    V222I

    Promoter

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214997

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (Tmem115 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCINeo-myc-Itch/2859

    Plasmid

    #11427

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Itch (Itch Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct

    Plasmid

    #175552

    Purpose

    Targeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    SOX2 (Sox2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    FKBPV-HA2-P2A-mCherry

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS mNotch1-IC (DM#270)

    Plasmid

    #15079

    Depositor

    Douglas Melton

    Article

    Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

    Insert

    Notch1-IC (Notch1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K1750A

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MoFGFR2c-ires neo

    Plasmid

    #86492

    Purpose

    mamalian cell expression of FGFR2c

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR2 IIIc 15D6 (Fgfr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSG5-HA-ERR3 S45A

    Plasmid

    #37849

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    Estrogen-related receptor gamma (Esrrg Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Serine 45 to Alanine (AGC to GCC)

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge